MetaCyc Pathway: Entner-Doudoroff pathway II (non-phosphorylative) in Klebsiella michiganensis M5al

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Reactions and Genes RCH2_defined_Glucose_noNitrogen in Eppendorf tube
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Glucose 1-dehydrogenase (NADP(+)):
D-glucopyranose + NADP+→D-glucono-1,5-lactone + NADPH + H+ (EC 1.1.1.119; 1.1.1.360)
No genes
Gluconolactonase:
D-glucono-1,5-lactone + H2O→D-gluconate + H+ (spontaneous) (EC 3.1.1.17)
No genes
D-gluconate→2-dehydro-3-deoxy-D-gluconate + H2O (EC 4.2.1.140; 4.2.1.39)
No genes
2-dehydro-3-deoxy-D-gluconate→D-glyceraldehyde + pyruvate (EC 4.1.2.51)
BWI76_RS26315 +0.2
D-glyceraldehyde + an oxidized electron carrier + H2O→D-glycerate + a reduced two electron carrier + H+ (EC 1.2.99.8)
BWI76_RS21985 +0.2
D-glycerate + ATP→2-phospho-D-glycerate + ADP + H+ (EC 2.7.1.165)
BWI76_RS07025 -0.3
BWI76_RS22820 -0.2
BWI76_RS24820 +0.1
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
BWI76_RS22790 N.D.
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
BWI76_RS16960 N.D.
BWI76_RS18115 +0.1
Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
BWI76_RS12730 -0.0
BWI76_RS18805 -1.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information