MetaCyc Pathway: ammonia assimilation cycle I in Pseudomonas fluorescens FW300-N2C3

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Reactions and Genes 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
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Glutamate--ammonia ligase:
ammonium + L-glutamate + ATP→L-glutamine + ADP + H+ + phosphate (EC 6.3.1.2)
AO356_01510 +0.2
AO356_04435 -0.1
AO356_09705 N.D.
AO356_13135 -3.5
AO356_13145 -3.4
AO356_18875 -0.1
AO356_30085 -0.1
Glutamate synthase (NADH) (in reverse):
L-glutamine + 2-oxoglutarate + NADH + H+→2 L-glutamate + NAD+ (EC 1.4.1.14)
AO356_09370 -0.2
AO356_23090 +0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information