MetaCyc Pathway: 2-methyl-branched fatty acid β-oxidation in Pseudomonas fluorescens FW300-N2C3

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Reactions and Genes 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
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Butyrate--CoA ligase:
a short-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)
No genes
a long-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a long-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
AO356_17045 -0.1
Butyrate--CoA ligase:
a medium-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)
No genes
a short-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a short-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
AO356_26355 -0.1
a medium-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a medium-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
AO356_09020 -0.2
AO356_14165 +0.2
AO356_14185 +0.0
AO356_14190 +0.0
AO356_15445 -1.0
AO356_17045 -0.1
Enoyl-CoA hydratase (in reverse):
a long-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a long-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
AO356_02330 N.D.
AO356_04640 -0.5
AO356_10120 +0.2
AO356_12440 -0.1
AO356_17825 +0.0
AO356_20400 +0.1
AO356_20420 N.D.
AO356_25890 +0.1
AO356_26360 -1.3
AO356_26370 +0.4
AO356_26645 +0.0
AO356_27920 +0.2
AO356_30295 -0.1
AO356_30355 -0.2
AO356_30360 +0.3
Enoyl-CoA hydratase (in reverse):
a short-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a short-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
AO356_02330 N.D.
AO356_04640 -0.5
AO356_10120 +0.2
AO356_12440 -0.1
AO356_17825 +0.0
AO356_20400 +0.1
AO356_20420 N.D.
AO356_25890 +0.1
AO356_26360 -1.3
AO356_26370 +0.4
AO356_26645 +0.0
AO356_27920 +0.2
AO356_30295 -0.1
AO356_30355 -0.2
AO356_30360 +0.3
Enoyl-CoA hydratase (in reverse):
a medium-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
AO356_02330 N.D.
AO356_04640 -0.5
AO356_10120 +0.2
AO356_12440 -0.1
AO356_17825 +0.0
AO356_20400 +0.1
AO356_20420 N.D.
AO356_25890 +0.1
AO356_26360 -1.3
AO356_26370 +0.4
AO356_26645 +0.0
AO356_27920 +0.2
AO356_30295 -0.1
AO356_30355 -0.2
AO356_30360 +0.3
3-hydroxyacyl-CoA dehydrogenase:
a long-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a long-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.35)
AO356_02330 N.D.
AO356_10120 +0.2
AO356_13845 +0.1
AO356_20400 +0.1
AO356_30295 -0.1
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
a short-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a short-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.178)
No genes
3-hydroxyacyl-CoA dehydrogenase:
a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a medium-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.35)
AO356_02330 N.D.
AO356_10120 +0.2
AO356_13845 +0.1
AO356_20400 +0.1
AO356_30295 -0.1
Acetyl-CoA C-acyltransferase (in reverse):
a long-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a long-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
AO356_02325 -0.6
AO356_03560 -0.2
AO356_04930 +0.1
AO356_08090 +0.0
AO356_20395 -0.2
AO356_21640 -0.5
AO356_26350 -0.7
Acetyl-CoA C-acyltransferase (in reverse):
a short-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
AO356_02325 -0.6
AO356_03560 -0.2
AO356_04930 +0.1
AO356_08090 +0.0
AO356_20395 -0.2
AO356_21640 -0.5
AO356_26350 -0.7
Acetyl-CoA C-acyltransferase (in reverse):
a medium-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
AO356_02325 -0.6
AO356_03560 -0.2
AO356_04930 +0.1
AO356_08090 +0.0
AO356_20395 -0.2
AO356_21640 -0.5
AO356_26350 -0.7

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information