MetaCyc Pathway: L-isoleucine biosynthesis I (from threonine) in Phaeobacter inhibens DSM 17395

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Reactions and Genes L-Valine (N)
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Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
PGA1_c34900 -0.3
PGA1_c36290 -0.1
PGA1_262p00750 +0.1
PGA1_c04590 -0.0
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
PGA1_c16600 +0.0
PGA1_c34900 -0.3
PGA1_c36290 -0.1
PGA1_262p00750 +0.1
PGA1_c04590 -0.0
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
PGA1_c16600 +0.0
PGA1_c34900 -0.3
PGA1_c36290 -0.1
PGA1_262p00750 +0.1
PGA1_c04590 -0.0
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
PGA1_c12350 -0.0
PGA1_c19360 +0.0
PGA1_c20210 -3.4
PGA1_c20220 +1.8
PGA1_262p02320 -0.2
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
PGA1_c06580 N.D.
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
PGA1_c29490 -3.7
PGA1_c07380 +0.1
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
PGA1_c34110 -0.9
PGA1_c34960 -0.3
PGA1_c04020 -0.9

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information