MetaCyc Pathway: biotin biosynthesis I in Ralstonia sp. UNC404CL21Col

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Reactions and Genes Sebacic (C)
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a malonyl-[acp] + S-adenosyl-L-methionine→a malonyl-[acp] methyl ester + S-adenosyl-L-homocysteine (EC 2.1.1.197)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a malonyl-[acp] + a malonyl-[acp] methyl ester + H+→a 3-oxoglutaryl-[acp] methyl ester + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
ABZR87_RS07225 -0.3
ABZR87_RS10155 N.D.
ABZR87_RS17840 +0.1
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxoglutaryl-[acp] methyl ester + NADPH + H+→a (3R)-3-hydroxyglutaryl-[acp] methyl ester + NADP+ (EC 1.1.1.100)
ABZR87_RS01455 +0.5
ABZR87_RS03540 -0.2
ABZR87_RS03590 +0.6
ABZR87_RS07820 -0.3
ABZR87_RS08385 +0.4
ABZR87_RS10145 N.D.
ABZR87_RS12060 -0.0
ABZR87_RS12565 +0.3
ABZR87_RS15380 +0.1
ABZR87_RS16115 +0.1
ABZR87_RS17625 -0.8
ABZR87_RS17640 -0.4
ABZR87_RS20660 N.D.
ABZR87_RS20680 +0.8
ABZR87_RS22225 -0.1
ABZR87_RS22525 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyglutaryl-[acp] methyl ester→a (2E)-enoylglutaryl-[acp] methyl ester + H2O (EC 4.2.1.59)
ABZR87_RS11880 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-enoylglutaryl-[acp] methyl ester + NADH + H+→a glutaryl-[acp] methyl ester + NAD+ (EC 1.3.1.9)
ABZR87_RS10675 N.D.
ABZR87_RS22515 +0.0
Beta-ketoacyl-acyl-carrier-protein synthase II:
a glutaryl-[acp] methyl ester + a malonyl-[acp] + H+→a 3-oxopimeloyl-[acp] methyl ester + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
ABZR87_RS07225 -0.3
ABZR87_RS10155 N.D.
ABZR87_RS17840 +0.1
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxopimeloyl-[acp] methyl ester + NADPH + H+→a (3R)-3-hydroxypimeloyl-[acp] methyl ester + NADP+ (EC 1.1.1.100)
ABZR87_RS01455 +0.5
ABZR87_RS03540 -0.2
ABZR87_RS03590 +0.6
ABZR87_RS07820 -0.3
ABZR87_RS08385 +0.4
ABZR87_RS10145 N.D.
ABZR87_RS12060 -0.0
ABZR87_RS12565 +0.3
ABZR87_RS15380 +0.1
ABZR87_RS16115 +0.1
ABZR87_RS17625 -0.8
ABZR87_RS17640 -0.4
ABZR87_RS20660 N.D.
ABZR87_RS20680 +0.8
ABZR87_RS22225 -0.1
ABZR87_RS22525 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxypimeloyl-[acp] methyl ester→a (2E)-enoylpimeloyl-[acp] methyl ester + H2O (EC 4.2.1.59)
ABZR87_RS11880 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-enoylpimeloyl-[acp] methyl ester + NADH + H+→a pimeloyl-[acp] methyl ester + NAD+ (EC 1.3.1.9)
ABZR87_RS10675 N.D.
ABZR87_RS22515 +0.0
a pimeloyl-[acp] methyl ester + H2O→a pimeloyl-[acp] + methanol + H+ (EC 3.1.1.85)
No genes
8-amino-7-oxononanoate synthase:
a pimeloyl-[acp] + L-alanine + H+→(8S)-8-amino-7-oxononanoate + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.47)
ABZR87_RS12340 -2.5
Adenosylmethionine--8-amino-7-oxononanoate transaminase:
(8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine→(7R,8S)-diaminononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate (EC 2.6.1.62)
ABZR87_RS04915 +0.1
ABZR87_RS12335 -2.6
ABZR87_RS21555 -0.4
Dethiobiotin synthase:
(7R,8S)-diaminononanoate + ATP + CO2→dethiobiotin + ADP + 3 H+ + phosphate (EC 6.3.3.3)
ABZR87_RS12345 -3.2
Biotin synthase:
dethiobiotin + H+ + 2 a reduced [2Fe-2S] ferredoxin + S-adenosyl-L-methionine + a sulfurated [sulfur carrier]→4,5-secobiotin + 5'-deoxyadenosine + L-methionine + 2 an oxidized [2Fe-2S] ferredoxin + an unsulfurated [sulfur carrier] (EC 2.8.1.6)
ABZR87_RS06265 -3.1
Biotin synthase:
4,5-secobiotin + S-adenosyl-L-methionine→biotin + 5'-deoxyadenosine + L-methionine + H+ (EC 2.8.1.6)
ABZR87_RS06265 -3.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information