MetaCyc Pathway: 2-methyl-branched fatty acid β-oxidation in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes 4-Hydroxybenzoic Acid (C)
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Butyrate--CoA ligase:
a short-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)
No genes
a long-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a long-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
Pf6N2E2_5333 +0.2
Butyrate--CoA ligase:
a medium-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)
No genes
a short-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a short-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
Pf6N2E2_1146 -0.5
a medium-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a medium-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
Pf6N2E2_1727 +0.1
Pf6N2E2_3686 -0.1
Pf6N2E2_4766 -2.1
Pf6N2E2_4768 -0.0
Pf6N2E2_4769 -0.2
Pf6N2E2_5026 +0.0
Pf6N2E2_5333 +0.2
Enoyl-CoA hydratase (in reverse):
a long-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a long-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
Pf6N2E2_1013 -0.0
Pf6N2E2_1046 +0.0
Pf6N2E2_1147 -0.8
Pf6N2E2_1149 +0.4
Pf6N2E2_1269 +0.3
Pf6N2E2_1833 -0.1
Pf6N2E2_1834 +0.2
Pf6N2E2_1835 +0.0
Pf6N2E2_1840 +0.2
Pf6N2E2_1850 +0.1
Pf6N2E2_1922 -0.3
Pf6N2E2_1934 -0.1
Pf6N2E2_1935 +0.3
Pf6N2E2_2290 N.D.
Pf6N2E2_2773 -0.1
Pf6N2E2_3894 -0.2
Pf6N2E2_5493 -0.0
Pf6N2E2_6000 -0.1
Pf6N2E2_6004 N.D.
Pf6N2E2_742 +0.2
Enoyl-CoA hydratase (in reverse):
a short-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a short-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
Pf6N2E2_1013 -0.0
Pf6N2E2_1046 +0.0
Pf6N2E2_1147 -0.8
Pf6N2E2_1149 +0.4
Pf6N2E2_1269 +0.3
Pf6N2E2_1833 -0.1
Pf6N2E2_1834 +0.2
Pf6N2E2_1835 +0.0
Pf6N2E2_1840 +0.2
Pf6N2E2_1850 +0.1
Pf6N2E2_1922 -0.3
Pf6N2E2_1934 -0.1
Pf6N2E2_1935 +0.3
Pf6N2E2_2290 N.D.
Pf6N2E2_2773 -0.1
Pf6N2E2_3894 -0.2
Pf6N2E2_5493 -0.0
Pf6N2E2_6000 -0.1
Pf6N2E2_6004 N.D.
Pf6N2E2_742 +0.2
Enoyl-CoA hydratase (in reverse):
a medium-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
Pf6N2E2_1013 -0.0
Pf6N2E2_1046 +0.0
Pf6N2E2_1147 -0.8
Pf6N2E2_1149 +0.4
Pf6N2E2_1269 +0.3
Pf6N2E2_1833 -0.1
Pf6N2E2_1834 +0.2
Pf6N2E2_1835 +0.0
Pf6N2E2_1840 +0.2
Pf6N2E2_1850 +0.1
Pf6N2E2_1922 -0.3
Pf6N2E2_1934 -0.1
Pf6N2E2_1935 +0.3
Pf6N2E2_2290 N.D.
Pf6N2E2_2773 -0.1
Pf6N2E2_3894 -0.2
Pf6N2E2_5493 -0.0
Pf6N2E2_6000 -0.1
Pf6N2E2_6004 N.D.
Pf6N2E2_742 +0.2
3-hydroxyacyl-CoA dehydrogenase:
a long-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a long-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.35)
Pf6N2E2_1833 -0.1
Pf6N2E2_1850 +0.1
Pf6N2E2_1922 -0.3
Pf6N2E2_2290 N.D.
Pf6N2E2_3894 -0.2
Pf6N2E2_4690 -0.3
Pf6N2E2_6000 -0.1
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
a short-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a short-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.178)
No genes
3-hydroxyacyl-CoA dehydrogenase:
a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a medium-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.35)
Pf6N2E2_1833 -0.1
Pf6N2E2_1850 +0.1
Pf6N2E2_1922 -0.3
Pf6N2E2_2290 N.D.
Pf6N2E2_3894 -0.2
Pf6N2E2_4690 -0.3
Pf6N2E2_6000 -0.1
Acetyl-CoA C-acyltransferase (in reverse):
a long-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a long-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
Pf6N2E2_1145 N.D.
Pf6N2E2_1675 +0.2
Pf6N2E2_1836 -0.0
Pf6N2E2_1851 +0.0
Pf6N2E2_2113 -0.0
Pf6N2E2_2289 N.D.
Pf6N2E2_2539 +0.0
Pf6N2E2_2835 -5.8
Pf6N2E2_3492 +0.0
Pf6N2E2_5999 -0.4
Acetyl-CoA C-acyltransferase (in reverse):
a short-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
Pf6N2E2_1145 N.D.
Pf6N2E2_1675 +0.2
Pf6N2E2_1836 -0.0
Pf6N2E2_1851 +0.0
Pf6N2E2_2113 -0.0
Pf6N2E2_2289 N.D.
Pf6N2E2_2539 +0.0
Pf6N2E2_2835 -5.8
Pf6N2E2_3492 +0.0
Pf6N2E2_5999 -0.4
Acetyl-CoA C-acyltransferase (in reverse):
a medium-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
Pf6N2E2_1145 N.D.
Pf6N2E2_1675 +0.2
Pf6N2E2_1836 -0.0
Pf6N2E2_1851 +0.0
Pf6N2E2_2113 -0.0
Pf6N2E2_2289 N.D.
Pf6N2E2_2539 +0.0
Pf6N2E2_2835 -5.8
Pf6N2E2_3492 +0.0
Pf6N2E2_5999 -0.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information