MetaCyc Pathway: (S)-lactate fermentation to propanoate, acetate and hydrogen in Bacteroides thetaiotaomicron VPI-5482

Reactions and Genes	BHIS with Trimethoprim 0.00625 mM
L-lactate dehydrogenase: (S)-lactate + NAD+→pyruvate + NADH + H+ (EC 1.1.1.27)
No genes
Pyruvate carboxylase: pyruvate + ATP + hydrogen carbonate→oxaloacetate + ADP + H+ + phosphate (EC 6.4.1.1)
BT1196	+0.5
BT1449	+0.2
BT1697	-0.2
BT1915	-0.5
Formate C-acetyltransferase (in reverse): pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
BT1055	+0.2
BT2955	-0.1
BT2956	-0.3
BT4737	-1.1
BT4738	-1.8
formate + H+→H2 + CO2
No genes
Malate dehydrogenase (in reverse): oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
BT2510	+0.2
BT3911	+1.2
Phosphate acetyltransferase: acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
BT3692	N.D.
Acetate kinase (in reverse): acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
BT0704	+1.8
BT3693	N.D.
Fumarate hydratase: (S)-malate→fumarate + H2O (EC 4.2.1.2)
BT2256	N.D.
Succinate dehydrogenase (ubiquinone): fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
No genes
succinate + propanoyl-CoA→succinyl-CoA + propanoate (EC 2.8.3.27)
BT3193	-0.6
Methylmalonyl-CoA mutase (in reverse): succinyl-CoA→(R)-methylmalonyl-CoA (EC 5.4.99.2)
BT2090	-0.5
BT2091	-0.5
Methylmalonyl-CoA epimerase: (R)-methylmalonyl-CoA→(S)-methylmalonyl-CoA (EC 5.1.99.1)
BT1685	N.D.
(S)-methylmalonyl-CoA[in] + Na+[in] + H+[out]→propanoyl-CoA[in] + CO2[in] + Na+[out] (EC 7.2.4.3)
BT1688	N.D.
BT1917	+0.0

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information