MetaCyc Pathway: assimilatory sulfate reduction I in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Trisodium citrate (C)
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Sulfate adenylyltransferase:
sulfate + ATP + H+→adenosine 5'-phosphosulfate + diphosphate
(EC 2.7.7.4)
Pf6N2E2_5257 -1.0
Pf6N2E2_5258 -1.2
Adenylyl-sulfate kinase:
adenosine 5'-phosphosulfate + ATP→3'-phosphoadenylyl-sulfate + ADP + H+
(EC 2.7.1.25)
Pf6N2E2_3654 -0.2
Pf6N2E2_5258 -1.2
Phosphoadenylyl-sulfate reductase (thioredoxin) (in reverse):
3'-phosphoadenylyl-sulfate + a reduced thioredoxin→sulfite + adenosine 3',5'-bisphosphate + an oxidized thioredoxin + 2 H+
(EC 1.8.4.8)
Pf6N2E2_6054 N.D.
Sulfite reductase (NADPH) (in reverse):
sulfite + 3 NADPH + 5 H+→hydrogen sulfide + 3 NADP+ + 3 H2O
(EC 1.8.1.2)
Pf6N2E2_1511 +0.1
Pf6N2E2_2073 -3.4
Pf6N2E2_3335 -1.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information