MetaCyc Pathway: 4-methylphenol degradation to protocatechuate in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Trisodium citrate (C)
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4-methylphenol + 2 an oxidized azurin + H2O→4-hydroxybenzyl alcohol + 2 H+ + 2 a reduced azurin (EC 1.17.9.1)
No genes
4-hydroxybenzyl alcohol + 2 an oxidized azurin→4-hydroxybenzaldehyde + 2 H+ + 2 a reduced azurin (EC 1.17.9.1)
No genes
4-hydroxybenzaldehyde + NADP+ + H2O→4-hydroxybenzoate + NADPH + 2 H+ (EC 1.2.1.96)
Pf6N2E2_225 +0.4
4-hydroxybenzoate 3-monooxygenase:
4-hydroxybenzoate + NADPH + dioxygen + H+→protocatechuate + NADP+ + H2O
(EC 1.14.13.2)
Pf6N2E2_2914 -2.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information