MetaCyc Pathway: protocatechuate degradation II (ortho-cleavage pathway) in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Trisodium citrate (C)
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Protocatechuate 3,4-dioxygenase:
protocatechuate + dioxygen→3-carboxy-cis,cis-muconate + 2 H+ (EC 1.13.11.3)
Pf6N2E2_2833 -1.7
Pf6N2E2_2834 -2.1
3-carboxy-cis,cis-muconate cycloisomerase (in reverse):
3-carboxy-cis,cis-muconate + H+→(2-carboxy-2,5-dihydro-5-oxofuran-2-yl)-acetate (EC 5.5.1.2)
Pf6N2E2_2831 -1.8
4-carboxymuconolactone decarboxylase:
(2-carboxy-2,5-dihydro-5-oxofuran-2-yl)-acetate + H+→(4,5-dihydro-5-oxofuran-2-yl)-acetate + CO2 (EC 4.1.1.44)
Pf6N2E2_2829 -1.7
Pf6N2E2_3293 +0.8
3-oxoadipate enol-lactonase:
(4,5-dihydro-5-oxofuran-2-yl)-acetate + H2O→3-oxoadipate + H+ (EC 3.1.1.24)
Pf6N2E2_2401 -0.0
Pf6N2E2_2683 -0.0
Pf6N2E2_2830 -2.5

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information