MetaCyc Pathway: NAD de novo biosynthesis II (from tryptophan) in Xanthomonas campestris pv. campestris strain 8004

Reactions and Genes	Citric Acid 16.67 mM (C)
Tryptophan 2,3-dioxygenase: L-tryptophan + dioxygen→N-formyl-L-kynurenine (EC 1.13.11.11; 1.13.11.52)
Xcc-8004.3754.1	+0.5
Xcc-8004.3755.1	-0.6
Xcc-8004.573.1	-1.2
Arylformamidase: N-formyl-L-kynurenine + H2O→L-kynurenine + formate + H+ (EC 3.5.1.9)
No genes
Kynurenine 3-monooxygenase: L-kynurenine + NADPH + dioxygen + H+→3-hydroxy-L-kynurenine + NADP+ + H2O (EC 1.14.13.9)
Xcc-8004.3328.1	-0.6
Kynureninase: 3-hydroxy-L-kynurenine + H2O→3-hydroxyanthranilate + L-alanine + H+ (EC 3.7.1.3)
Xcc-8004.3327.1	-0.3
3-hydroxyanthranilate 3,4-dioxygenase: 3-hydroxyanthranilate + dioxygen→2-amino-3-carboxymuconate-6-semialdehyde (EC 1.13.11.6)
Xcc-8004.3325.1	-0.8
2-amino-3-carboxymuconate-6-semialdehyde→quinolinate + H+ + H2O (spontaneous)
Xcc-8004.3325.1	-0.8
Nicotinate-nucleotide diphosphorylase (carboxylating) (in reverse): quinolinate + 2 H+ + 5-phospho-α-D-ribose 1-diphosphate→β-nicotinate D-ribonucleotide + CO2 + diphosphate (EC 2.4.2.19)
Xcc-8004.2009.1	-0.9
Nicotinate-nucleotide adenylyltransferase: β-nicotinate D-ribonucleotide + ATP + H+→nicotinate adenine dinucleotide + diphosphate (EC 2.7.7.18)
Xcc-8004.1873.1	-2.7
NAD(+) synthase (glutamine-hydrolyzing): nicotinate adenine dinucleotide + ATP + L-glutamine + H2O→NAD+ + AMP + L-glutamate + diphosphate + H+ (EC 6.3.5.1)
Xcc-8004.1359.1	-1.2

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information