MetaCyc Pathway: methylaspartate cycle in Burkholderia phytofirmans PsJN

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Reactions and Genes 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
BPHYT_RS10160 +0.2
BPHYT_RS30145 +0.0
BPHYT_RS33980 +1.3
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
BPHYT_RS10160 +0.2
BPHYT_RS30145 +0.0
BPHYT_RS33980 +1.3
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→2-oxoglutarate + CO2 + NADPH (EC 1.1.1.42)
BPHYT_RS15730 -0.8
BPHYT_RS20300 N.D.
Glutamate dehydrogenase (in reverse):
2-oxoglutarate + ammonium + NADH + H+→L-glutamate + NAD+ + H2O (EC 1.4.1.2)
BPHYT_RS16705 N.D.
BPHYT_RS22475 -0.3
Methylaspartate mutase (in reverse):
L-glutamate→(2S, 3S)-3-methylaspartate (EC 5.4.99.1)
No genes
Methylaspartate ammonia-lyase:
(2S, 3S)-3-methylaspartate→mesaconate + ammonium (EC 4.3.1.2)
No genes
mesaconate + succinyl-CoA→2-methylfumaryl-CoA + succinate (EC 2.8.3.26)
No genes
2-methylfumaryl-CoA + H2O→(2R,3S)-β-methylmalyl-CoA (EC 4.2.1.148)
No genes
Malyl-CoA lyase:
(2R,3S)-β-methylmalyl-CoA→glyoxylate + propanoyl-CoA (EC 4.1.3.24)
No genes
Malyl-CoA lyase (in reverse):
glyoxylate + acetyl-CoA→(S)-malyl-CoA (EC 4.1.3.24)
No genes
Propionyl-CoA carboxylase:
propanoyl-CoA + ATP + hydrogen carbonate→(S)-methylmalonyl-CoA + ADP + H+ + phosphate (EC 6.4.1.3)
BPHYT_RS12420 N.D.
BPHYT_RS33860 N.D.
Methylmalonyl-CoA epimerase (in reverse):
(S)-methylmalonyl-CoA→(R)-methylmalonyl-CoA (EC 5.1.99.1)
No genes
(S)-malyl-CoA + H2O→(S)-malate + coenzyme A + H+ (EC 3.1.2.30)
No genes
Methylmalonyl-CoA mutase:
(R)-methylmalonyl-CoA→succinyl-CoA (EC 5.4.99.2)
BPHYT_RS02085 -0.1
Succinate--CoA ligase (ADP-forming) (in reverse):
succinyl-CoA + ADP + phosphate→succinate + ATP + coenzyme A (EC 6.2.1.5)
BPHYT_RS16340 -1.3
BPHYT_RS16345 -1.2
Succinate dehydrogenase (ubiquinone):
succinate[in] + an electron-transfer quinone→fumarate[in] + an electron-transfer quinol (EC 1.3.5.1)
BPHYT_RS33935 N.D.
Fumarate hydratase (in reverse):
fumarate + H2O→(S)-malate (EC 4.2.1.2)
BPHYT_RS06985 +0.4
BPHYT_RS14615 +0.4
Malate dehydrogenase:
(S)-malate + NAD+→oxaloacetate + NADH + H+ (EC 1.1.1.37; 1.1.1.38)
BPHYT_RS27110 +0.3
BPHYT_RS28460 +0.7
BPHYT_RS33955 N.D.
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
BPHYT_RS15810 +0.1
BPHYT_RS19980 -0.6
BPHYT_RS20940 -0.1
BPHYT_RS27440 -0.1
BPHYT_RS33920 +2.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information