MetaCyc Pathway: partial TCA cycle (obligate autotrophs) in Burkholderia phytofirmans PsJN

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Reactions and Genes 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
BPHYT_RS05225 -3.1
Citrate (Si)-synthase:
oxaloacetate + acetyl-CoA + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
BPHYT_RS15810 +0.1
BPHYT_RS19980 -0.6
BPHYT_RS20940 -0.1
BPHYT_RS27440 -0.1
BPHYT_RS33920 +2.0
oxaloacetate[in] + an electron-transfer quinol→(S)-malate[in] + an electron-transfer quinone (EC 1.1.5.4)
No genes
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
BPHYT_RS10160 +0.2
BPHYT_RS30145 +0.0
BPHYT_RS33980 +1.3
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
BPHYT_RS06985 +0.4
BPHYT_RS14615 +0.4
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
BPHYT_RS10160 +0.2
BPHYT_RS30145 +0.0
BPHYT_RS33980 +1.3
Succinate dehydrogenase (ubiquinone) (in reverse):
fumarate[in] + an electron-transfer quinol→succinate[in] + an electron-transfer quinone (EC 1.3.5.1)
BPHYT_RS33935 N.D.
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→2-oxoglutarate + CO2 + NADPH (EC 1.1.1.42)
BPHYT_RS15730 -0.8
BPHYT_RS20300 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information