MetaCyc Pathway: L-isoleucine degradation I in Phaeobacter inhibens DSM 17395

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Reactions and Genes m.b. Doxycycline hyclate 0.002 mg/ml
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Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)
PGA1_c34110 -0.1
PGA1_c34960 +0.0
PGA1_c04020 -0.0
2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD+→(2S)-2-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
No genes
(E)-2-methylcrotonoyl-CoA + H2O→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (EC 4.2.1.150)
PGA1_c12020 +0.2
PGA1_c14440 -0.0
PGA1_c15660 +0.1
PGA1_c16990 -1.5
PGA1_c22740 -0.0
PGA1_c23460 -0.1
PGA1_c26820 -0.2
PGA1_c36500 N.D.
PGA1_262p01540 -0.1
PGA1_262p01850 -0.1
PGA1_262p01980 -0.0
PGA1_c04110 -0.1
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+→2-methylacetoacetyl-CoA + NADH + H+ (EC 1.1.1.178)
PGA1_c11320 +0.0
PGA1_c12020 +0.2
PGA1_c22740 -0.0
PGA1_c04110 -0.1
PGA1_c08420 +0.1
Acetyl-CoA C-acyltransferase (in reverse):
2-methylacetoacetyl-CoA + coenzyme A→acetyl-CoA + propanoyl-CoA (EC 2.3.1.16)
PGA1_c12030 +0.3
PGA1_c22730 +0.1
PGA1_c33180 -0.0
PGA1_c03400 +0.1
PGA1_c34350 +0.1
PGA1_c04090 -0.3
PGA1_c04120 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information