MetaCyc Pathway: pyruvate fermentation to butanoate in Herbaspirillum seropedicae SmR1

Add experiment(s):


Reactions and Genes L-Malic (C) with ammonium chloride; anaerobic; 8 days
remove
Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
HSERO_RS01180 +2.7
HSERO_RS01265 +0.8
HSERO_RS04635 +0.0
HSERO_RS19070 -0.0
HSERO_RS19990 +0.0
HSERO_RS20030 -0.1
HSERO_RS20660 -0.0
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
HSERO_RS01260 +0.3
HSERO_RS04630 -0.0
HSERO_RS05890 -0.1
HSERO_RS12745 +0.2
HSERO_RS20035 -0.0
HSERO_RS22630 +0.0
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
HSERO_RS01260 +0.3
HSERO_RS01270 +0.2
HSERO_RS12745 +0.2
HSERO_RS18840 -0.1
HSERO_RS19405 -0.1
HSERO_RS20020 -0.3
HSERO_RS20640 +0.1
HSERO_RS20665 +0.0
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Phosphate butyryltransferase:
butanoyl-CoA + phosphate→butanoyl phosphate + coenzyme A (EC 2.3.1.19)
HSERO_RS01300 -1.3
Branched-chain-fatty-acid kinase (in reverse):
butanoyl phosphate + ADP→butanoate + ATP (EC 2.7.2.14; 2.7.2.7)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information