MetaCyc Pathway: 3-methylbutanol biosynthesis (engineered) in Pseudomonas stutzeri RCH2

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Reactions and Genes Def 2-Furfuraldehyde 0.032 vol%
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2-isopropylmalate synthase:
3-methyl-2-oxobutanoate + acetyl-CoA + H2O→(2S)-2-isopropylmalate + coenzyme A + H+ (EC 2.3.3.13)
Psest_1056 -0.7
Psest_1277 -1.0
3-isopropylmalate dehydratase (in reverse):
(2S)-2-isopropylmalate→2-isopropylmaleate + H2O (EC 4.2.1.33)
Psest_2590 -4.3
Psest_2591 -3.0
3-isopropylmalate dehydratase (in reverse):
2-isopropylmaleate + H2O→(2R,3S)-3-isopropylmalate (EC 4.2.1.33)
Psest_2590 -4.3
Psest_2591 -3.0
3-isopropylmalate dehydrogenase:
(2R,3S)-3-isopropylmalate + NAD+→(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ (EC 1.1.1.85)
Psest_2589 -3.1
3-isopropylmalate dehydrogenase:
(2S)-2-isopropyl-3-oxosuccinate + H+→4-methyl-2-oxopentanoate + CO2 (spontaneous) (EC 1.1.1.85)
Psest_2589 -3.1
Branched-chain-2-oxoacid decarboxylase:
4-methyl-2-oxopentanoate + H+→3-methylbutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
3-methylbutanal + NADH + H+→3-methylbutanol + NAD+ (EC 1.1.1.1)
Psest_1276 +1.9
Psest_1434 +0.2
Psest_2086 +0.5
Psest_2268 +0.1
Psest_2816 +0.3
Psest_3782 -0.0
Psest_3967 +0.2
Psest_4317 -0.1
Psest_0672 -0.1
Psest_0890 -0.4
Psest_0931 +0.9

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information