MetaCyc Pathway: thiazole component of thiamine diphosphate biosynthesis I in Pseudomonas stutzeri RCH2

Reactions and Genes	Def Benzalkonium Chloride 0.002 mg/ml
1-deoxy-D-xylulose-5-phosphate synthase: D-glyceraldehyde 3-phosphate + H+ + pyruvate→1-deoxy-D-xylulose 5-phosphate + CO2 (EC 2.2.1.7)
Psest_0641	N.D.
L-tyrosine + NADPH + S-adenosyl-L-methionine→2-iminoacetate + 5'-deoxyadenosine + 4-methylphenol + L-methionine + NADP+ + H+ (EC 4.1.99.19)
No genes
Cysteine desulfurase: L-cysteine + a [ThiI sulfur-carrier protein]-L-cysteine→a [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + L-alanine (EC 2.8.1.7)
Psest_1251	N.D.
Psest_1464	+1.2
Psest_1699	-0.5
a [ThiS sulfur-carrier protein] + ATP + H+→a carboxy-adenylated-[ThiS sulfur-carrier protein] + diphosphate (EC 2.7.7.73)
No genes
a carboxy-adenylated-[ThiS sulfur-carrier protein] + a [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + 2 a reduced ferredoxin [iron-sulfur] cluster→a thiocarboxylated-[ThiS sulfur-carrier protein] + AMP + 2 an oxidized ferredoxin [iron-sulfur] cluster + a [ThiI sulfur-carrier protein]-L-cysteine
Psest_3927	+0.3
2-iminoacetate + 1-deoxy-D-xylulose 5-phosphate + a thiocarboxylated-[ThiS sulfur-carrier protein]→a [ThiS sulfur-carrier protein] + 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 2 H2O (EC 2.8.1.10)
Psest_0267	-3.9

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information