MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Pseudomonas stutzeri RCH2

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Reactions and Genes Def Nalidixic 0.001 mg/ml
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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
Psest_1036 N.D.
Psest_1037 -2.0
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
Psest_1038 N.D.
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
Psest_0234 N.D.
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
Psest_1276 -0.1
Psest_1434 -0.1
Psest_2086 -0.2
Psest_2268 +1.4
Psest_2816 +0.3
Psest_3782 +0.0
Psest_3967 -0.0
Psest_4317 +0.0
Psest_0672 +0.4
Psest_0890 +0.2
Psest_0931 +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information