MetaCyc Pathway: L-isoleucine degradation I in Mycobacterium tuberculosis H37Rv

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Reactions and Genes 7H9 with nitrite 1.5 mM; pH 5.5
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Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)
Rv2210c N.D.
2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD+→(2S)-2-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
Rv2500c -1.8
(E)-2-methylcrotonoyl-CoA + H2O→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (EC 4.2.1.150)
Rv0222 +0.2
Rv0456c -0.2
Rv0632c +0.4
Rv0675 -0.2
Rv0860 N.D.
Rv0905 +0.3
Rv1070c +0.1
Rv1136 N.D.
Rv1141c +0.3
Rv1142c +0.1
Rv1472 +0.2
Rv1935c +0.2
Rv2486 +0.3
Rv2679 -0.2
Rv2831 -0.3
Rv3039c +0.3
Rv3373 N.D.
Rv3374 -0.1
Rv3516 +0.0
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+→2-methylacetoacetyl-CoA + NADH + H+ (EC 1.1.1.178)
Rv0860 N.D.
Rv1144 +0.2
Acetyl-CoA C-acyltransferase (in reverse):
2-methylacetoacetyl-CoA + coenzyme A→acetyl-CoA + propanoyl-CoA (EC 2.3.1.16)
Rv0243 +0.1
Rv0859 N.D.
Rv0914c +0.4
Rv1074c -1.2
Rv1135A N.D.
Rv1323 -0.7
Rv2790c +0.1
Rv3523 +0.1
Rv3546 -0.4
Rv3556c +0.5

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information