MetaCyc Pathway: (8E,10E)-dodeca-8,10-dienol biosynthesis in Pseudomonas fluorescens FW300-N2C3

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Reactions and Genes 4C survival; 1152 hrs
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palmitoyl-CoA[in] + an oxidized electron-transfer flavoprotein[in] + H+[in]→(2E)-hexadec-2-enoyl-CoA[in] + a reduced electron-transfer flavoprotein[in] (EC 1.3.8.9)
No genes
(8E,10E)-dodeca-8,10-dienoate + 2 NADPH + 3 H+→(8E,10E)-dodeca-8,10-dienol + 2 NADP+ + H2O
No genes
Enoyl-CoA hydratase (in reverse):
(2E)-hexadec-2-enoyl-CoA + H2O→(S)-3-hydroxyhexadecanoyl-CoA (EC 4.2.1.17)
AO356_02330 N.D.
AO356_04640 +0.0
AO356_10120 -0.1
AO356_12440 +0.0
AO356_17825 +0.2
AO356_20400 +0.3
AO356_20420 N.D.
AO356_25890 -0.7
AO356_26360 -1.4
AO356_26370 +0.3
AO356_26645 -1.0
AO356_27920 +1.6
AO356_30295 -0.1
AO356_30355 -0.2
AO356_30360 -0.1
Long-chain-3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxyhexadecanoyl-CoA + NAD+→3-oxopalmitoyl-CoA + NADH + H+ (EC 1.1.1.211)
AO356_02330 N.D.
AO356_10120 -0.1
AO356_20400 +0.3
AO356_30295 -0.1
Acetyl-CoA C-myristoyltransferase (in reverse):
3-oxopalmitoyl-CoA + coenzyme A→myristoyl-CoA + acetyl-CoA (EC 2.3.1.155)
AO356_03560 +0.2
AO356_26350 +0.5
myristoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-tetradec-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
No genes
Enoyl-CoA hydratase (in reverse):
(2E)-tetradec-2-enoyl-CoA + H2O→(S)-3-hydroxytetradecanoyl-CoA (EC 4.2.1.17)
AO356_02330 N.D.
AO356_04640 +0.0
AO356_10120 -0.1
AO356_12440 +0.0
AO356_17825 +0.2
AO356_20400 +0.3
AO356_20420 N.D.
AO356_25890 -0.7
AO356_26360 -1.4
AO356_26370 +0.3
AO356_26645 -1.0
AO356_27920 +1.6
AO356_30295 -0.1
AO356_30355 -0.2
AO356_30360 -0.1
(S)-3-hydroxytetradecanoyl-CoA + NAD+→3-oxomyristoyl-CoA + NADH + H+
AO356_02330 N.D.
AO356_10120 -0.1
AO356_20400 +0.3
AO356_30295 -0.1
Acetyl-CoA C-acyltransferase (in reverse):
3-oxomyristoyl-CoA + coenzyme A→lauroyl-CoA + acetyl-CoA (EC 2.3.1.16)
AO356_02325 +0.1
AO356_03560 +0.2
AO356_04930 +0.4
AO356_08090 -0.1
AO356_20395 +0.2
AO356_21640 +0.4
AO356_26350 +0.5
lauroyl-CoA + 2 a ferrocytochrome b5 + dioxygen + 2 H+→(9E)-dodecenoyl-CoA + 2 a ferricytochrome b5 + 2 H2O
No genes
(9E)-dodecenoyl-CoA + 2 a ferrocytochrome b5 + dioxygen + 2 H+→(8E,10E)-dodeca-8,10-dienoyl-CoA + 2 a ferricytochrome b5 + 2 H2O
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information