MetaCyc Pathway: L-isoleucine degradation I in Herbaspirillum seropedicae SmR1

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Reactions and Genes Carbon source Phenylacetic 5 mM
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Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)
HSERO_RS03330 -0.0
2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD+→(2S)-2-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
No genes
(E)-2-methylcrotonoyl-CoA + H2O→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (EC 4.2.1.150)
HSERO_RS01260 -0.5
HSERO_RS01270 +0.0
HSERO_RS12745 +0.4
HSERO_RS18840 -0.1
HSERO_RS19405 -0.0
HSERO_RS20020 +0.2
HSERO_RS20640 -4.9
HSERO_RS20665 -4.7
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+→2-methylacetoacetyl-CoA + NADH + H+ (EC 1.1.1.178)
HSERO_RS01260 -0.5
HSERO_RS04630 -0.5
HSERO_RS05565 -0.2
HSERO_RS05890 +0.1
HSERO_RS12745 +0.4
HSERO_RS20035 -0.4
HSERO_RS22630 -0.1
Acetyl-CoA C-acyltransferase (in reverse):
2-methylacetoacetyl-CoA + coenzyme A→acetyl-CoA + propanoyl-CoA (EC 2.3.1.16)
HSERO_RS01180 -0.0
HSERO_RS01265 -0.3
HSERO_RS04635 +0.4
HSERO_RS19070 -0.2
HSERO_RS19990 -0.0
HSERO_RS20030 +0.1
HSERO_RS20660 -4.7

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information