MetaCyc Pathway: superpathway of purine nucleotides de novo biosynthesis I in Klebsiella michiganensis M5al

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Reactions and Genes Cobalt chloride 0.5 mM
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Amidophosphoribosyltransferase (in reverse):
5-phospho-α-D-ribose 1-diphosphate + L-glutamine + H2O→5-phospho-β-D-ribosylamine + L-glutamate + diphosphate (EC 2.4.2.14)
BWI76_RS20350 +0.2
Phosphoribosylamine--glycine ligase:
5-phospho-β-D-ribosylamine + glycine + ATP→N1-(5-phospho-β-D-ribosyl)glycinamide + ADP + H+ + phosphate (EC 6.3.4.13)
BWI76_RS01610 +0.2
Phosphoribosylglycinamide formyltransferase:
N1-(5-phospho-β-D-ribosyl)glycinamide + an N10-formyltetrahydrofolate→N2-formyl-N1-(5-phospho-β-D-ribosyl)glycinamide + H+ + a tetrahydrofolate (EC 2.1.2.2)
BWI76_RS18090 +0.2
BWI76_RS20970 +0.3
Phosphoribosylformylglycinamidine synthase:
N2-formyl-N1-(5-phospho-β-D-ribosyl)glycinamide + ATP + L-glutamine + H2O→2-(formamido)-N1-(5-phospho-β-D-ribosyl)acetamidine + ADP + L-glutamate + H+ + phosphate (EC 6.3.5.3)
BWI76_RS21265 -0.4
Phosphoribosylformylglycinamidine cyclo-ligase:
2-(formamido)-N1-(5-phospho-β-D-ribosyl)acetamidine + ATP→5-amino-1-(5-phospho-β-D-ribosyl)imidazole + ADP + H+ + phosphate (EC 6.3.3.1)
BWI76_RS20965 +0.1
Phosphoribosylaminoimidazole carboxylase (in reverse):
5-amino-1-(5-phospho-β-D-ribosyl)imidazole + CO2→5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + 2 H+ (EC 4.1.1.21)
BWI76_RS07065 -0.2
BWI76_RS07070 -0.1
Phosphoribosylaminoimidazolesuccinocarboxamide synthase:
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate→5'-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole + ADP + H+ + phosphate (EC 6.3.2.6)
BWI76_RS20895 +0.3
Adenylosuccinate lyase:
5'-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole→5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + fumarate (EC 4.3.2.2)
BWI76_RS11305 N.D.
Phosphoribosylaminoimidazolecarboxamide formyltransferase:
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + an N10-formyltetrahydrofolate→5-formamido-1-(5-phospho-D-ribosyl)-imidazole-4-carboxamide + a tetrahydrofolate (EC 2.1.2.3)
BWI76_RS01615 +0.3
IMP cyclohydrolase (in reverse):
5-formamido-1-(5-phospho-D-ribosyl)-imidazole-4-carboxamide→IMP + H2O (EC 3.5.4.10)
BWI76_RS01615 +0.3
Adenylosuccinate synthase:
IMP + L-aspartate + GTP→adenylosuccinate + GDP + 2 H+ + phosphate (EC 6.3.4.4)
BWI76_RS02565 N.D.
IMP dehydrogenase:
IMP + NAD+ + H2O→XMP + NADH + H+ (EC 1.1.1.205)
BWI76_RS21030 N.D.
Adenylosuccinate lyase:
adenylosuccinate→AMP + fumarate (EC 4.3.2.2)
BWI76_RS11305 N.D.
GMP synthase (glutamine-hydrolyzing):
XMP + ATP + L-glutamine + H2O→GMP + AMP + L-glutamate + diphosphate + 2 H+ (EC 6.3.5.2)
BWI76_RS14000 +0.8
BWI76_RS21025 N.D.
Adenylate kinase:
AMP + ATP→2 ADP (EC 2.7.4.3)
BWI76_RS06840 N.D.
Guanylate kinase:
GMP + ATP→GDP + ADP (EC 2.7.4.8)
BWI76_RS02190 -0.2
BWI76_RS27420 N.D.
Ribonucleoside-diphosphate reductase (in reverse):
ADP + a reduced thioredoxin→dADP + an oxidized thioredoxin + H2O (EC 1.17.4.1)
BWI76_RS19940 N.D.
BWI76_RS19945 N.D.
BWI76_RS22050 -1.9
BWI76_RS22055 -1.7
BWI76_RS22060 -0.8
Nucleoside-diphosphate kinase:
GDP + ATP→GTP + ADP (EC 2.7.4.6)
BWI76_RS06840 N.D.
BWI76_RS21100 +0.5
Ribonucleoside-diphosphate reductase (in reverse):
GDP + a reduced thioredoxin→dGDP + an oxidized thioredoxin + H2O (EC 1.17.4.1)
BWI76_RS19940 N.D.
BWI76_RS19945 N.D.
BWI76_RS22050 -1.9
BWI76_RS22055 -1.7
BWI76_RS22060 -0.8
Nucleoside-diphosphate kinase:
dADP + ATP→ADP + dATP (EC 2.7.4.6)
BWI76_RS21100 +0.5
Nucleoside-diphosphate kinase:
dGDP + ATP→dGTP + ADP (EC 2.7.4.6)
BWI76_RS06840 N.D.
BWI76_RS21100 +0.5

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information