MetaCyc Pathway: superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis in Klebsiella michiganensis M5al

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Reactions and Genes Cobalt chloride 0.5 mM
remove
Phosphoglucomutase (in reverse):
D-glucopyranose 6-phosphate→α-D-glucopyranose 1-phosphate
(EC 5.4.2.2)
BWI76_RS08290 N.D.
BWI76_RS25130 N.D.
Glucose-1-phosphate thymidylyltransferase:
α-D-glucopyranose 1-phosphate + H+ + dTTP→dTDP-α-D-glucose + diphosphate
(EC 2.7.7.24)
BWI76_RS01105 -1.6
BWI76_RS19060 -0.3
dTDP-glucose 4,6-dehydratase:
dTDP-α-D-glucose→dTDP-4-dehydro-6-deoxy-α-D-glucopyranose + H2O
(EC 4.2.1.46)
BWI76_RS18995 N.D.
BWI76_RS19065 -0.5
dTDP-4-dehydro-6-deoxyglucose reductase (in reverse):
dTDP-4-dehydro-6-deoxy-α-D-glucopyranose + NADPH + H+→dTDP-α-D-fucopyranose + NADP+
(EC 1.1.1.266)
No genes
dTDP-4-amino-4,6-dideoxy-D-glucose transaminase (in reverse):
dTDP-4-dehydro-6-deoxy-α-D-glucopyranose + L-glutamate→dTDP-viosamine + 2-oxoglutarate
(EC 2.6.1.33)
No genes
dTDP-4-dehydrorhamnose 3,5-epimerase:
dTDP-4-dehydro-6-deoxy-α-D-glucopyranose→dTDP-4-dehydro-β-L-rhamnose
(EC 5.1.3.13)
BWI76_RS19055 -2.0
dTDP-4-amino-4,6-dideoxygalactose transaminase (in reverse):
dTDP-4-dehydro-6-deoxy-α-D-glucopyranose + L-glutamate→dTDP-4-amino-4,6-dideoxy-α-D-galactose + 2-oxoglutarate
(EC 2.6.1.59)
BWI76_RS01115 -4.6
dTDP-4-dehydro-6-deoxy-α-D-glucopyranose→dTDP-3-dehydro-6-deoxy-α-D-galactopyranose (EC 5.3.2.3)
No genes
dTDP-4-dehydro-6-deoxy-α-D-glucopyranose→dTDP-3-dehydro-6-deoxy-α-D-glucopyranose (EC 5.3.2.4)
No genes
dTDP-4-dehydro-6-deoxyglucose reductase (in reverse):
dTDP-4-dehydro-6-deoxy-α-D-glucopyranose + NAD(P)H + H+→dTDP-α-D-fucopyranose + NAD(P)+
(EC 1.1.1.266)
No genes
dTDP-4-dehydrorhamnose reductase (in reverse):
dTDP-4-dehydro-β-L-rhamnose + NADPH + H+→dTDP-β-L-rhamnose + NADP+
(EC 1.1.1.133)
BWI76_RS19050 -1.6
dTDP-3-dehydro-6-deoxy-α-D-glucopyranose + L-glutamate→dTDP-3-amino-3,6-dideoxy-α-D-glucopyranose + 2-oxoglutarate (EC 2.6.1.89)
No genes
dTDP-3-dehydro-6-deoxy-α-D-galactopyranose + L-glutamate→dTDP-3-amino-3,6-dideoxy-α-D-galactopyranose + 2-oxoglutarate (EC 2.6.1.90)
No genes
dTDP-4-dehydro-β-L-rhamnose + NADH + H+→dTDP-β-L-pneumose + NAD+ (EC 1.1.1.339)
BWI76_RS19050 -1.6
dTDP-viosamine + acetyl-CoA→dTDP-N-acetylviosamine + coenzyme A + H+ (EC 2.3.1.209)
No genes
dTDP-α-D-fucopyranose→dTDP-β-D-fucofuranose (EC 5.4.99.59)
BWI76_RS18975 -1.1
dTDP-4-amino-4,6-dideoxy-α-D-galactose + acetyl-CoA→dTDP-4-acetamido-α-D-fucose + coenzyme A + H+ (EC 2.3.1.210)
BWI76_RS01110 -4.7
dTDP-3-amino-3,6-dideoxy-α-D-galactopyranose + acetyl-CoA→dTDP-3-acetamido-3-deoxy-α-D-fucose + coenzyme A + H+ (EC 2.3.1.197)
No genes
dTDP-3-amino-3,6-dideoxy-α-D-glucopyranose + acetyl-CoA→dTDP-3-acetamido-3,6-dideoxy-α-D-glucopyranose + coenzyme A + H+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information