MetaCyc Pathway: gluconeogenesis III in Klebsiella michiganensis M5al

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Reactions and Genes Nickel (II) chloride 1.5 mM
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Pyruvate carboxylase:
pyruvate + ATP + hydrogen carbonate→oxaloacetate + ADP + H+ + phosphate (EC 6.4.1.1)
No genes
Malate dehydrogenase:
(S)-malate + NAD+→oxaloacetate + NADH + H+ (EC 1.1.1.37; 1.1.1.38)
BWI76_RS13830 +0.2
BWI76_RS16775 +0.1
BWI76_RS25420 -0.5
Phosphoenolpyruvate carboxykinase (GTP):
oxaloacetate + GTP→phosphoenolpyruvate + CO2 + GDP (EC 4.1.1.32)
No genes
Phosphopyruvate hydratase (in reverse):
phosphoenolpyruvate + H2O→2-phospho-D-glycerate (EC 4.2.1.11)
BWI76_RS22790 N.D.
2-phospho-D-glycerate→3-phospho-D-glycerate (EC 5.4.2.11)
BWI76_RS08605 -4.5
Phosphoglycerate kinase:
3-phospho-D-glycerate + ATP→3-phospho-D-glyceroyl phosphate + ADP (EC 2.7.2.3)
BWI76_RS23980 N.D.
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (in reverse):
3-phospho-D-glyceroyl phosphate + NADH + H+→D-glyceraldehyde 3-phosphate + NAD+ + phosphate (EC 1.2.1.12)
BWI76_RS11505 N.D.
BWI76_RS12975 -0.2
BWI76_RS13215 -0.7
Triose-phosphate isomerase:
D-glyceraldehyde 3-phosphate→glycerone phosphate (EC 5.3.1.1)
BWI76_RS03100 +0.2
BWI76_RS27465 N.D.
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-glyceraldehyde 3-phosphate→β-D-fructofuranose 1,6-bisphosphate (EC 4.1.2.13)
BWI76_RS00545 +0.1
BWI76_RS14880 -0.3
BWI76_RS19370 -2.0
BWI76_RS23975 N.D.
BWI76_RS27560 +0.3
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
BWI76_RS02815 -0.6
BWI76_RS06160 -0.1
BWI76_RS08660 +0.2
BWI76_RS27445 +0.0
Glucose-6-phosphate isomerase (in reverse):
β-D-fructofuranose 6-phosphate→α-D-glucose 6-phosphate (EC 5.3.1.9)
BWI76_RS01800 -2.6
Sugar-terminal-phosphatase:
D-glucopyranose 6-phosphate + H2O→D-glucopyranose + phosphate (EC 3.1.3.58; 3.1.3.9)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information