MetaCyc Pathway: superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis in Klebsiella michiganensis M5al

Add experiment(s):


Reactions and Genes Nickel (II) chloride 1.5 mM
remove
Carbamoyl-phosphate synthase (glutamine-hydrolyzing):
L-glutamine + hydrogen carbonate + 2 ATP + H2O→carbamoyl phosphate + 2 ADP + L-glutamate + 2 H+ + phosphate (EC 6.3.5.5)
BWI76_RS04465 +0.1
BWI76_RS04470 -0.3
Aspartate carbamoyltransferase:
carbamoyl phosphate + L-aspartate→N-carbamoyl-L-aspartate + H+ + phosphate (EC 2.1.3.2)
BWI76_RS02990 -0.2
BWI76_RS02995 -0.5
Dihydroorotase (in reverse):
N-carbamoyl-L-aspartate + H+→(S)-dihydroorotate + H2O (EC 3.5.2.3)
BWI76_RS07015 +0.1
BWI76_RS10990 +1.6
Dihydroorotate dehydrogenase:
(S)-dihydroorotate[in] + an electron-transfer quinone→orotate[in] + an electron-transfer quinol (EC 1.3.5.2)
BWI76_RS09910 +0.2
Orotate phosphoribosyltransferase (in reverse):
orotate + 5-phospho-α-D-ribose 1-diphosphate→orotidine 5'-phosphate + diphosphate (EC 2.4.2.10)
BWI76_RS27385 +0.1
Orotidine-5'-phosphate decarboxylase:
orotidine 5'-phosphate + H+→UMP + CO2 (EC 4.1.1.23)
BWI76_RS11965 +0.3
Cytidylate kinase:
UMP + ATP→UDP + ADP (EC 2.7.4.14; 2.7.4.22)
BWI76_RS05220 N.D.
BWI76_RS09775 N.D.
Nucleoside-diphosphate kinase:
UDP + ATP→UTP + ADP (EC 2.7.4.6)
BWI76_RS06840 N.D.
BWI76_RS21100 +0.2
Ribonucleoside-diphosphate reductase (in reverse):
UDP + a reduced thioredoxin→dUDP + an oxidized thioredoxin + H2O (EC 1.17.4.1)
BWI76_RS19940 N.D.
BWI76_RS19945 N.D.
BWI76_RS22050 -0.9
BWI76_RS22055 -1.3
BWI76_RS22060 -1.2
CTP synthase:
UTP + L-glutamine + ATP + H2O→CTP + ADP + L-glutamate + 2 H+ + phosphate (EC 6.3.4.2)
BWI76_RS10250 -0.0
BWI76_RS11445 +0.4
BWI76_RS22795 N.D.
Nucleoside-diphosphate kinase:
dUDP + ATP→dUTP + ADP (EC 2.7.4.6)
BWI76_RS21100 +0.2
dUTP diphosphatase:
dUTP + H2O→dUMP + diphosphate + H+ (EC 3.6.1.23; 3.6.1.9)
BWI76_RS20020 -0.0
BWI76_RS27375 N.D.
Nucleoside-triphosphatase:
CTP + H2O→CDP + H+ + phosphate (EC 3.6.1.15; 3.6.1.5)
BWI76_RS24115 +0.2
Ribonucleoside-diphosphate reductase (in reverse):
CDP + a reduced thioredoxin→dCDP + an oxidized thioredoxin + H2O (EC 1.17.4.1)
BWI76_RS19940 N.D.
BWI76_RS19945 N.D.
BWI76_RS22050 -0.9
BWI76_RS22055 -1.3
BWI76_RS22060 -1.2
Thymidylate synthase:
dUMP + a 5,10-methylenetetrahydrofolate→dTMP + a 7,8-dihydrofolate (EC 2.1.1.45)
BWI76_RS14320 -0.7
Nucleoside-diphosphate kinase:
dCDP + ATP→dCTP + ADP (EC 2.7.4.6)
BWI76_RS06840 N.D.
BWI76_RS21100 +0.2
T(2)-induced deoxynucleotide kinase:
dTMP + ATP→dTDP + ADP (EC 2.7.4.12; 2.7.4.13; 2.7.4.9)
BWI76_RS11115 N.D.
Nucleoside-diphosphate kinase:
dTDP + ATP→dTTP + ADP (EC 2.7.4.6)
BWI76_RS06840 N.D.
BWI76_RS21100 +0.2

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information