MetaCyc Pathway: superpathway of guanosine nucleotides de novo biosynthesis II in Klebsiella michiganensis M5al

Reactions and Genes	Nickel (II) chloride 1.5 mM
IMP dehydrogenase: IMP + NAD+ + H2O→XMP + NADH + H+ (EC 1.1.1.205)
BWI76_RS21030	N.D.
GMP synthase (glutamine-hydrolyzing): XMP + ATP + L-glutamine + H2O→GMP + AMP + L-glutamate + diphosphate + 2 H+ (EC 6.3.5.2)
BWI76_RS14000	+0.4
BWI76_RS21025	N.D.
Guanylate kinase: GMP + ATP→GDP + ADP (EC 2.7.4.8)
BWI76_RS02190	-0.3
BWI76_RS27420	N.D.
Nucleoside-diphosphate kinase: GDP + ATP→GTP + ADP (EC 2.7.4.6)
BWI76_RS06840	N.D.
BWI76_RS21100	+0.2
Ribonucleoside-diphosphate reductase (in reverse): GDP + a reduced thioredoxin→dGDP + an oxidized thioredoxin + H2O (EC 1.17.4.1)
BWI76_RS19940	N.D.
BWI76_RS19945	N.D.
BWI76_RS22050	-0.9
BWI76_RS22055	-1.3
BWI76_RS22060	-1.2
Ribonucleoside-diphosphate reductase (in reverse): GDP + a reduced NrdH glutaredoxin-like protein→dGDP + an oxidized NrdH glutaredoxin-like protein + H2O (EC 1.17.4.1)
BWI76_RS19940	N.D.
BWI76_RS19945	N.D.
BWI76_RS22055	-1.3
BWI76_RS22060	-1.2
Nucleoside-diphosphate kinase: dGDP + ATP→dGTP + ADP (EC 2.7.4.6)
BWI76_RS06840	N.D.
BWI76_RS21100	+0.2
GTP + formate + H+→dGTP + CO2 + H2O (EC 1.1.98.6)
BWI76_RS02935	+0.1

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information