MetaCyc Pathway: formaldehyde assimilation II (assimilatory RuMP Cycle) in Klebsiella michiganensis M5al

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Reactions and Genes Nickel (II) chloride 1.5 mM
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6-phosphofructokinase:
β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H+ (EC 2.7.1.11)
BWI76_RS00530 +0.0
BWI76_RS00735 N.D.
BWI76_RS17175 N.D.
3-hexulose-6-phosphate synthase (in reverse):
formaldehyde + D-ribulose 5-phosphate→hexulose 6-phosphate (EC 4.1.2.43)
No genes
Fructose-bisphosphate aldolase:
β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)
BWI76_RS00545 +0.1
BWI76_RS14880 -0.3
BWI76_RS19370 -2.0
BWI76_RS23975 N.D.
BWI76_RS27560 +0.3
6-phospho-3-hexuloisomerase:
hexulose 6-phosphate→β-D-fructofuranose 6-phosphate (EC 5.3.1.27)
No genes
Transketolase (in reverse):
β-D-fructofuranose 6-phosphate + D-glyceraldehyde 3-phosphate→D-erythrose 4-phosphate + D-xylulose 5-phosphate (EC 2.2.1.1)
BWI76_RS07680 +0.0
BWI76_RS07685 N.D.
BWI76_RS08905 -0.1
BWI76_RS08910 +0.3
BWI76_RS09705 -0.4
BWI76_RS09710 -0.1
BWI76_RS20245 -0.1
BWI76_RS20250 -0.4
BWI76_RS20835 +0.0
BWI76_RS24015 N.D.
Transaldolase (in reverse):
D-erythrose 4-phosphate + β-D-fructofuranose 6-phosphate→D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate (EC 2.2.1.2)
BWI76_RS00820 -0.0
BWI76_RS04270 -0.4
Transketolase:
D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate→D-xylulose 5-phosphate + D-ribose 5-phosphate (EC 2.2.1.1)
BWI76_RS07680 +0.0
BWI76_RS07685 N.D.
BWI76_RS08905 -0.1
BWI76_RS08910 +0.3
BWI76_RS09705 -0.4
BWI76_RS09710 -0.1
BWI76_RS20245 -0.1
BWI76_RS20250 -0.4
BWI76_RS20835 +0.0
BWI76_RS24015 N.D.
Ribose-5-phosphate isomerase:
D-ribose 5-phosphate→D-ribulose 5-phosphate (EC 5.3.1.6)
BWI76_RS02175 -0.1
BWI76_RS23925 N.D.
Ribulose-phosphate 3-epimerase (in reverse):
D-xylulose 5-phosphate→D-ribulose 5-phosphate (EC 5.1.3.1)
BWI76_RS02150 +0.0
BWI76_RS23675 -0.2
BWI76_RS26070 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information