MetaCyc Pathway: superpathway of histidine, purine, and pyrimidine biosynthesis in Klebsiella michiganensis M5al

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Reactions and Genes Nickel (II) chloride 1.5 mM
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Carbamoyl-phosphate synthase (glutamine-hydrolyzing):
L-glutamine + hydrogen carbonate + 2 ATP + H2O→carbamoyl phosphate + 2 ADP + L-glutamate + 2 H+ + phosphate (EC 6.3.5.5)
BWI76_RS04465 +0.1
BWI76_RS04470 -0.3
Ribose-phosphate diphosphokinase:
D-ribose 5-phosphate + ATP→5-phospho-α-D-ribose 1-diphosphate + AMP + H+ (EC 2.7.6.1)
BWI76_RS17460 N.D.
BWI76_RS25955 +0.2
Aspartate carbamoyltransferase:
carbamoyl phosphate + L-aspartate→N-carbamoyl-L-aspartate + H+ + phosphate (EC 2.1.3.2)
BWI76_RS02990 -0.2
BWI76_RS02995 -0.5
ATP phosphoribosyltransferase (in reverse):
5-phospho-α-D-ribose 1-diphosphate + ATP→1-(5-phospho-β-D-ribosyl)-ATP + diphosphate (EC 2.4.2.17)
BWI76_RS18920 -0.5
Amidophosphoribosyltransferase (in reverse):
5-phospho-α-D-ribose 1-diphosphate + L-glutamine + H2O→5-phospho-β-D-ribosylamine + L-glutamate + diphosphate (EC 2.4.2.14)
BWI76_RS20350 -0.0
Dihydroorotase (in reverse):
N-carbamoyl-L-aspartate + H+→(S)-dihydroorotate + H2O (EC 3.5.2.3)
BWI76_RS07015 +0.1
BWI76_RS10990 +1.6
Phosphoribosylamine--glycine ligase:
5-phospho-β-D-ribosylamine + ATP + glycine→N1-(5-phospho-β-D-ribosyl)glycinamide + ADP + H+ + phosphate (EC 6.3.4.13)
BWI76_RS01610 +0.1
Phosphoribosyl-ATP diphosphatase:
1-(5-phospho-β-D-ribosyl)-ATP + H2O→1-(5-phospho-β-D-ribosyl)-AMP + diphosphate + H+ (EC 3.6.1.31)
BWI76_RS18955 -0.3
Dihydroorotate dehydrogenase:
(S)-dihydroorotate[in] + an electron-transfer quinone→orotate[in] + an electron-transfer quinol (EC 1.3.5.2)
BWI76_RS09910 +0.2
N1-(5-phospho-β-D-ribosyl)glycinamide + ATP + formate→N2-formyl-N1-(5-phospho-β-D-ribosyl)glycinamide + ADP + H+ + phosphate (EC 6.3.1.21)
BWI76_RS18090 -0.2
Phosphoribosyl-AMP cyclohydrolase:
1-(5-phospho-β-D-ribosyl)-AMP + H2O→1-(5-phospho-β-D-ribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide (EC 3.5.4.19)
BWI76_RS18955 -0.3
Phosphoribosylformylglycinamidine synthase:
N2-formyl-N1-(5-phospho-β-D-ribosyl)glycinamide + L-glutamine + ATP + H2O→2-(formamido)-N1-(5-phospho-β-D-ribosyl)acetamidine + ADP + L-glutamate + H+ + phosphate (EC 6.3.5.3)
BWI76_RS21265 -0.1
Orotate phosphoribosyltransferase (in reverse):
orotate + 5-phospho-α-D-ribose 1-diphosphate→orotidine 5'-phosphate + diphosphate (EC 2.4.2.10)
BWI76_RS27385 +0.1
isomerase:
1-(5-phospho-β-D-ribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide→phosphoribulosylformimino-AICAR-phosphate (EC 5.3.1.16)
BWI76_RS18945 N.D.
Phosphoribosylformylglycinamidine cyclo-ligase:
2-(formamido)-N1-(5-phospho-β-D-ribosyl)acetamidine + ATP→5-amino-1-(5-phospho-β-D-ribosyl)imidazole + ADP + H+ + phosphate (EC 6.3.3.1)
BWI76_RS20965 +0.1
phosphoribulosylformimino-AICAR-phosphate + L-glutamine→5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)-glycerol 3-phosphate + L-glutamate + H+ (EC 4.3.2.10)
BWI76_RS18940 -1.3
BWI76_RS18950 -1.7
Orotidine-5'-phosphate decarboxylase:
orotidine 5'-phosphate + H+→UMP + CO2 (EC 4.1.1.23)
BWI76_RS11965 +0.3
Imidazoleglycerol-phosphate dehydratase:
D-erythro-1-(imidazol-4-yl)-glycerol 3-phosphate→3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O (EC 4.2.1.19)
BWI76_RS18935 -2.3
Cytidylate kinase:
UMP + ATP→UDP + ADP (EC 2.7.4.14; 2.7.4.22)
BWI76_RS05220 N.D.
BWI76_RS09775 N.D.
5-(carboxyamino)imidazole ribonucleotide synthase:
5-amino-1-(5-phospho-β-D-ribosyl)imidazole + hydrogen carbonate + ATP→N5-carboxyaminoimidazole ribonucleotide + ADP + 2 H+ + phosphate (EC 6.3.4.18)
BWI76_RS07065 -0.0
Histidinol-phosphate transaminase (in reverse):
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate→L-histidinol phosphate + 2-oxoglutarate (EC 2.6.1.9)
BWI76_RS18930 -1.1
5-(carboxyamino)imidazole ribonucleotide mutase:
N5-carboxyaminoimidazole ribonucleotide→5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate (EC 5.4.99.18)
BWI76_RS07070 +0.1
Nucleoside-diphosphate kinase:
UDP + ATP→UTP + ADP (EC 2.7.4.6)
BWI76_RS06840 N.D.
BWI76_RS21100 +0.2
CTP synthase:
UTP + L-glutamine + ATP + H2O→CTP + ADP + L-glutamate + 2 H+ + phosphate (EC 6.3.4.2)
BWI76_RS10250 -0.0
BWI76_RS11445 +0.4
BWI76_RS22795 N.D.
Histidinol-phosphatase:
L-histidinol phosphate + H2O→histidinol + phosphate (EC 3.1.3.15)
BWI76_RS06160 -0.1
BWI76_RS18935 -2.3
Phosphoribosylaminoimidazolesuccinocarboxamide synthase:
L-aspartate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP→5'-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole + ADP + H+ + phosphate (EC 6.3.2.6)
BWI76_RS20895 +0.3
Adenylosuccinate lyase:
5'-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole→5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + fumarate (EC 4.3.2.2)
BWI76_RS11305 N.D.
Histidinol dehydrogenase:
histidinol + NAD+→histidinal + NADH + H+ (EC 1.1.1.23)
BWI76_RS18925 -1.4
Phosphoribosylaminoimidazolecarboxamide formyltransferase:
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + an N10-formyltetrahydrofolate→5-formamido-1-(5-phospho-D-ribosyl)-imidazole-4-carboxamide + a tetrahydrofolate (EC 2.1.2.3)
BWI76_RS01615 +0.3
Histidinol dehydrogenase:
histidinal + NAD+ + H2O→L-histidine + NADH + 2 H+ (EC 1.1.1.23)
BWI76_RS18925 -1.4
IMP cyclohydrolase (in reverse):
5-formamido-1-(5-phospho-D-ribosyl)-imidazole-4-carboxamide→IMP + H2O (EC 3.5.4.10)
BWI76_RS01615 +0.3
Adenylosuccinate synthase:
IMP + L-aspartate + GTP→adenylosuccinate + GDP + 2 H+ + phosphate (EC 6.3.4.4)
BWI76_RS02565 N.D.
IMP dehydrogenase:
IMP + NAD+ + H2O→XMP + NADH + H+ (EC 1.1.1.205)
BWI76_RS21030 N.D.
Adenylosuccinate lyase:
adenylosuccinate→AMP + fumarate (EC 4.3.2.2)
BWI76_RS11305 N.D.
GMP synthase (glutamine-hydrolyzing):
XMP + L-glutamine + ATP + H2O→GMP + AMP + L-glutamate + diphosphate + 2 H+ (EC 6.3.5.2)
BWI76_RS14000 +0.4
BWI76_RS21025 N.D.
Adenylate kinase:
AMP + ATP→2 ADP (EC 2.7.4.3)
BWI76_RS06840 N.D.
Guanylate kinase:
GMP + ATP→GDP + ADP (EC 2.7.4.8)
BWI76_RS02190 -0.3
BWI76_RS27420 N.D.
Ribonucleoside-diphosphate reductase (in reverse):
ADP + a reduced thioredoxin→dADP + an oxidized thioredoxin + H2O (EC 1.17.4.1)
BWI76_RS19940 N.D.
BWI76_RS19945 N.D.
BWI76_RS22050 -0.9
BWI76_RS22055 -1.3
BWI76_RS22060 -1.2
Nucleoside-diphosphate kinase:
GDP + ATP→GTP + ADP (EC 2.7.4.6)
BWI76_RS06840 N.D.
BWI76_RS21100 +0.2
Ribonucleoside-diphosphate reductase (in reverse):
GDP + a reduced thioredoxin→dGDP + an oxidized thioredoxin + H2O (EC 1.17.4.1)
BWI76_RS19940 N.D.
BWI76_RS19945 N.D.
BWI76_RS22050 -0.9
BWI76_RS22055 -1.3
BWI76_RS22060 -1.2
Ribonucleoside-diphosphate reductase (in reverse):
ADP + a reduced NrdH glutaredoxin-like protein→dADP + an oxidized NrdH glutaredoxin-like protein + H2O (EC 1.17.4.1)
BWI76_RS19940 N.D.
BWI76_RS19945 N.D.
BWI76_RS22055 -1.3
BWI76_RS22060 -1.2
Ribonucleoside-diphosphate reductase (in reverse):
GDP + a reduced NrdH glutaredoxin-like protein→dGDP + an oxidized NrdH glutaredoxin-like protein + H2O (EC 1.17.4.1)
BWI76_RS19940 N.D.
BWI76_RS19945 N.D.
BWI76_RS22055 -1.3
BWI76_RS22060 -1.2
Nucleoside-diphosphate kinase:
dADP + ATP→ADP + dATP (EC 2.7.4.6)
BWI76_RS21100 +0.2
Nucleoside-diphosphate kinase:
dGDP + ATP→dGTP + ADP (EC 2.7.4.6)
BWI76_RS06840 N.D.
BWI76_RS21100 +0.2
ATP + formate + H+→dATP + CO2 + H2O (EC 1.1.98.6)
BWI76_RS02935 +0.1
GTP + formate + H+→dGTP + CO2 + H2O (EC 1.1.98.6)
BWI76_RS02935 +0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information