MetaCyc Pathway: superpathway of N-acetylneuraminate degradation in Klebsiella michiganensis M5al

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Reactions and Genes Nickel (II) chloride 1.5 mM
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N-acetyl-β-neuraminate→aceneuramate (EC 5.1.3.24)
BWI76_RS00085 -0.1
N-acetylneuraminate lyase:
aceneuramate→N-acetyl-D-mannosamine + pyruvate (EC 4.1.3.3)
BWI76_RS00060 +0.1
BWI76_RS03425 -0.0
N-acylmannosamine kinase:
N-acetyl-D-mannosamine + ATP→N-acetyl-D-mannosamine 6-phosphate + ADP + H+ (EC 2.7.1.60)
BWI76_RS00080 +0.2
BWI76_RS04475 +0.2
BWI76_RS20960 +0.1
N-acylglucosamine-6-phosphate 2-epimerase:
N-acetyl-D-mannosamine 6-phosphate→N-acetyl-D-glucosamine 6-phosphate (EC 5.1.3.9)
BWI76_RS00075 +0.2
N-acetylglucosamine-6-phosphate deacetylase:
N-acetyl-D-glucosamine 6-phosphate + H2O→D-glucosamine 6-phosphate + acetate (EC 3.5.1.25)
BWI76_RS08210 -1.3
Glucosamine-6-phosphate deaminase:
D-glucosamine 6-phosphate + H2O→β-D-fructofuranose 6-phosphate + ammonium (EC 3.5.99.6)
BWI76_RS08215 -1.5
6-phosphofructokinase:
β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H+ (EC 2.7.1.11)
BWI76_RS00530 +0.0
BWI76_RS00735 N.D.
BWI76_RS17175 N.D.
Fructose-bisphosphate aldolase:
β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)
BWI76_RS00545 +0.1
BWI76_RS14880 -0.3
BWI76_RS19370 -2.0
BWI76_RS23975 N.D.
BWI76_RS27560 +0.3
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
BWI76_RS02815 -0.6
BWI76_RS06160 -0.1
BWI76_RS08660 +0.2
BWI76_RS27445 +0.0
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
BWI76_RS03100 +0.2
BWI76_RS27465 N.D.
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
BWI76_RS11505 N.D.
BWI76_RS12975 -0.2
BWI76_RS13215 -0.7
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
BWI76_RS23980 N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
BWI76_RS04200 +0.3
BWI76_RS08605 -4.5
BWI76_RS13025 +0.4
BWI76_RS27230 -0.1
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
BWI76_RS08605 -4.5
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
BWI76_RS22790 N.D.
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2)
BWI76_RS17065 +0.7
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
BWI76_RS16960 N.D.
BWI76_RS18115 +0.1
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
BWI76_RS03570 +0.0
BWI76_RS03575 +0.1
BWI76_RS09070 +0.2
BWI76_RS09075 +0.4
BWI76_RS09730 -0.9
BWI76_RS09735 -0.1
BWI76_RS10655 -0.0
BWI76_RS10660 +0.1
BWI76_RS21375 -0.5
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
BWI76_RS02835 -0.0
BWI76_RS03590 +0.2
BWI76_RS16905 +0.3
BWI76_RS17250 +0.7
BWI76_RS20790 -0.1
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
BWI76_RS03560 -0.2
BWI76_RS20240 N.D.
BWI76_RS20805 +0.1
BWI76_RS23150 -0.5
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
BWI76_RS18090 -0.2
BWI76_RS20235 N.D.
BWI76_RS20815 +0.1
BWI76_RS20820 +0.1
BWI76_RS23200 -0.4
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
BWI76_RS00645 -0.3
BWI76_RS02835 -0.0
BWI76_RS03140 -0.6
BWI76_RS03320 -0.0
BWI76_RS12395 -0.1
BWI76_RS13800 +0.3
BWI76_RS15240 -0.2
BWI76_RS17250 +0.7
BWI76_RS25510 +0.2
BWI76_RS27960 -0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information