MetaCyc Pathway: gluconeogenesis I in Klebsiella michiganensis M5al

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Reactions and Genes Nickel (II) chloride 1.5 mM
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Malate dehydrogenase (oxaloacetate-decarboxylating):
(S)-malate + NAD+→pyruvate + CO2 + NADH (EC 1.1.1.38)
BWI76_RS13830 +0.2
Malate dehydrogenase:
(S)-malate + NAD+→oxaloacetate + NADH + H+ (EC 1.1.1.37; 1.1.1.38)
BWI76_RS13830 +0.2
BWI76_RS16775 +0.1
BWI76_RS25420 -0.5
Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)):
(S)-malate + NADP+→pyruvate + CO2 + NADPH (EC 1.1.1.40)
BWI76_RS20830 -0.2
Phosphoenolpyruvate carboxykinase (ATP):
oxaloacetate + ATP→phosphoenolpyruvate + ADP + CO2 (EC 4.1.1.49)
BWI76_RS17605 -0.1
BWI76_RS26155 +0.3
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2)
BWI76_RS17065 +0.7
Phosphopyruvate hydratase (in reverse):
phosphoenolpyruvate + H2O→2-phospho-D-glycerate (EC 4.2.1.11)
BWI76_RS22790 N.D.
2-phospho-D-glycerate→3-phospho-D-glycerate (EC 5.4.2.12)
BWI76_RS04200 +0.3
BWI76_RS08605 -4.5
BWI76_RS13025 +0.4
BWI76_RS27230 -0.1
Phosphoglycerate kinase:
3-phospho-D-glycerate + ATP→3-phospho-D-glyceroyl phosphate + ADP (EC 2.7.2.3)
BWI76_RS23980 N.D.
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (in reverse):
3-phospho-D-glyceroyl phosphate + NADH + H+→D-glyceraldehyde 3-phosphate + NAD+ + phosphate (EC 1.2.1.12)
BWI76_RS11505 N.D.
BWI76_RS12975 -0.2
BWI76_RS13215 -0.7
Fructose-bisphosphate aldolase (in reverse):
D-glyceraldehyde 3-phosphate + glycerone phosphate→β-D-fructofuranose 1,6-bisphosphate (EC 4.1.2.13)
BWI76_RS00545 +0.1
BWI76_RS14880 -0.3
BWI76_RS19370 -2.0
BWI76_RS23975 N.D.
BWI76_RS27560 +0.3
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
BWI76_RS02815 -0.6
BWI76_RS06160 -0.1
BWI76_RS08660 +0.2
BWI76_RS27445 +0.0
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
BWI76_RS03100 +0.2
BWI76_RS27465 N.D.
Glucose-6-phosphate isomerase (in reverse):
β-D-fructofuranose 6-phosphate→α-D-glucose 6-phosphate (EC 5.3.1.9)
BWI76_RS01800 -2.6

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information