MetaCyc Pathway: anandamide biosynthesis II in Pseudomonas fluorescens FW300-N2E2

Add experiment(s):


Reactions and Genes Nickel (II) chloride 0.0025 M
remove
Glycerol-3-phosphate O-acyltransferase:
arachidonoyl-CoA + sn-glycerol 3-phosphate→1-arachidinoyl-sn-glycerol 3-phosphate + coenzyme A (EC 2.3.1.15)
Pf6N2E2_3087 -0.4
Pf6N2E2_4795 +2.8
Pf6N2E2_5950 -0.5
1-acylglycerol-3-phosphate O-acyltransferase:
1-arachidinoyl-sn-glycerol 3-phosphate + an acyl-CoA→a 1-arachidonoyl-2-acyl-sn-glycerol 3-phosphate + coenzyme A (EC 2.3.1.51)
Pf6N2E2_2816 N.D.
Pf6N2E2_4192 N.D.
Pf6N2E2_4950 -0.2
Pf6N2E2_5765 -0.3
Pf6N2E2_6003 +0.0
Phosphatidate phosphatase:
a 1-arachidonoyl-2-acyl-sn-glycerol 3-phosphate + H2O→a 1-arachidonoyl-2-acyl-sn-glycerol + phosphate (EC 3.1.3.4)
No genes
Diacylglycerol cholinephosphotransferase:
a 1-arachidonoyl-2-acyl-sn-glycerol + CDP-choline→a 1-arachidonoyl-2-acyl-sn-glycero-3-phosphocholine + CMP + H+ (EC 2.7.8.2)
No genes
a 1-arachidonoyl-2-acyl-sn-glycero-3-phosphocholine + a plasmenylethanolamine→an N-(arachidonoyl)ethanolamine plasmalogen + a 1-lysophosphatidylcholine + H+ (EC 2.3.1.M82)
No genes
Phospholipase A(2):
an N-(arachidonoyl)ethanolamine plasmalogen + H2O→a 2-lyso-(N-arachidonoyl)ethanolamine plasmalogen + a fatty acid + H+ (EC 3.1.1.4)
No genes
an N-(arachidonoyl)ethanolamine plasmalogen + H2O→anandamide + a 2-acyl-1-Z-alk-1-enyl-sn-glycerol 3-phosphoate + H+ (EC 3.1.4.54)
No genes
Alkylglycerophosphoethanolamine phosphodiesterase:
a 2-lyso-(N-arachidonoyl)ethanolamine plasmalogen + H2O→anandamide + a 1-O-(alk-1-enyl)-sn-3-phosphoglycerol + H+ (EC 3.1.4.39)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information