MetaCyc Pathway: C4 photosynthetic carbon assimilation cycle, NAD-ME type in Pseudomonas fluorescens FW300-N2C3

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Reactions and Genes Minimal media pH9
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Carbonate dehydratase (in reverse):
CO2 + H2O→hydrogen carbonate + H+ (EC 4.2.1.1)
AO356_11145 -0.5
AO356_16270 -2.4
AO356_23780 -0.5
Phosphoenolpyruvate carboxylase (in reverse):
hydrogen carbonate + phosphoenolpyruvate→oxaloacetate + phosphate (EC 4.1.1.31)
AO356_06185 -0.1
Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
AO356_01275 +0.0
AO356_03090 +0.1
AO356_03985 +0.2
AO356_06565 +0.0
AO356_12070 -0.1
AO356_12595 +0.1
AO356_16875 -0.8
AO356_19495 -0.1
AO356_19865 -0.2
AO356_19905 -0.1
AO356_20345 -0.2
AO356_22880 -0.1
AO356_24005 +0.0
AO356_24115 -0.1
AO356_24720 -0.0
AO356_24975 -0.1
AO356_25355 +0.3
L-aspartate[in]→L-aspartate[out]
No genes
Aspartate transaminase:
L-aspartate + 2-oxoglutarate→oxaloacetate + L-glutamate (EC 2.6.1.1)
AO356_01275 +0.0
AO356_03090 +0.1
AO356_03985 +0.2
AO356_06565 +0.0
AO356_12070 -0.1
AO356_12595 +0.1
AO356_16875 -0.8
AO356_19495 -0.1
AO356_19865 -0.2
AO356_19905 -0.1
AO356_20345 -0.2
AO356_22880 -0.1
AO356_24005 +0.0
AO356_24115 -0.1
AO356_24720 -0.0
AO356_24975 -0.1
AO356_25355 +0.3
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
No genes
Malate dehydrogenase (oxaloacetate-decarboxylating):
(S)-malate + NAD+→CO2 + pyruvate + NADH (EC 1.1.1.38)
No genes
Alanine transaminase (in reverse):
pyruvate + L-glutamate→L-alanine + 2-oxoglutarate (EC 2.6.1.2)
AO356_03090 +0.1
AO356_05050 -0.2
L-alanine[in]→L-alanine[out]
No genes
Alanine transaminase (in reverse):
L-alanine + 2-oxoglutarate→pyruvate + L-glutamate (EC 2.6.1.2)
AO356_03090 +0.1
Pyruvate, phosphate dikinase:
pyruvate + ATP + phosphate→phosphoenolpyruvate + AMP + diphosphate + H+ (EC 2.7.9.1)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information