MetaCyc Pathway: L-isoleucine degradation I in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes 4C survival; 672 hrs
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Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)
AO353_26655 -0.5
2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD+→(2S)-2-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
AO353_25670 +0.0
AO353_25680 +0.1
(E)-2-methylcrotonoyl-CoA + H2O→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (EC 4.2.1.150)
AO353_00510 -1.5
AO353_01155 +0.2
AO353_03780 +0.2
AO353_17555 -0.1
AO353_19125 N.D.
AO353_23055 +0.1
AO353_24830 -0.1
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+→2-methylacetoacetyl-CoA + NADH + H+ (EC 1.1.1.178)
AO353_00510 -1.5
AO353_01155 +0.2
AO353_25690 N.D.
Acetyl-CoA C-acyltransferase (in reverse):
2-methylacetoacetyl-CoA + coenzyme A→acetyl-CoA + propanoyl-CoA (EC 2.3.1.16)
AO353_00505 -1.8
AO353_01065 +4.5
AO353_01160 +0.3
AO353_05785 +0.0
AO353_13880 -0.4
AO353_17205 +0.0
AO353_25685 +4.3
AO353_27530 -0.1
AO353_27945 +0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information