MetaCyc Pathway: butanol and isobutanol biosynthesis (engineered) in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Hans 1000x minerals
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Glycine oxidase:
glycine + dioxygen + H2O→glyoxylate + ammonium + hydrogen peroxide (EC 1.4.3.19; 1.4.3.3)
Pf6N2E2_4186 +0.1
Pf6N2E2_5120 -0.2
Pf6N2E2_680 +0.2
3-ethylmalate synthase:
glyoxylate + butanoyl-CoA + H2O→3-ethylmalate + coenzyme A + H+ (EC 2.3.3.7)
No genes
3-isopropylmalate dehydrogenase:
3-ethylmalate + NAD+→2-oxovalerate + CO2 + NADH (EC 1.1.1.85)
Pf6N2E2_66 -0.1
2-oxovalerate→3-methyl-2-oxobutanoate
No genes
Pyruvate decarboxylase:
2-oxovalerate + H+→1-butanal + CO2 (EC 4.1.1.1)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
Pf6N2E2_1380 N.D.
Pf6N2E2_1522 +0.4
Pf6N2E2_194 +0.0
Pf6N2E2_2802 +0.1
Pf6N2E2_3044 -1.6
Pf6N2E2_4819 -0.0
Pf6N2E2_5980 -0.1
Pf6N2E2_840 +0.3
Pf6N2E2_999 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information