MetaCyc Pathway: superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation in Pseudomonas fluorescens FW300-N2E2

Reactions and Genes	Hans 1000x minerals
Pyruvate synthase: pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Acetaldehyde dehydrogenase (acetylating) (in reverse): acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
Pf6N2E2_1380	N.D.
Acetyl-CoA C-acyltransferase: 2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
Pf6N2E2_1145	N.D.
Pf6N2E2_1675	-0.0
Pf6N2E2_1836	+0.0
Pf6N2E2_1851	+0.2
Pf6N2E2_1923	+0.1
Pf6N2E2_2113	+0.1
Pf6N2E2_2289	N.D.
Pf6N2E2_2539	-0.1
Pf6N2E2_2835	-0.4
Pf6N2E2_3492	-0.1
Pf6N2E2_5999	-0.0
Phosphate acetyltransferase: acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
Pf6N2E2_5260	-0.2
Acetate kinase (in reverse): acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
Pf6N2E2_5444	-0.5
Alcohol dehydrogenase (in reverse): acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
Pf6N2E2_1380	N.D.
Pf6N2E2_1522	+0.4
Pf6N2E2_194	+0.0
Pf6N2E2_2802	+0.1
Pf6N2E2_3044	-1.6
Pf6N2E2_4819	-0.0
Pf6N2E2_5980	-0.1
Pf6N2E2_840	+0.3
Pf6N2E2_999	-0.1
3-hydroxyacyl-CoA dehydrogenase (in reverse): acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
Pf6N2E2_1722	+0.1
Pf6N2E2_1833	+0.0
Pf6N2E2_1850	+0.1
Pf6N2E2_1922	+0.2
Pf6N2E2_2290	N.D.
Pf6N2E2_3894	+0.2
Pf6N2E2_4690	+0.1
Pf6N2E2_6000	-0.3
Butyrate--acetoacetate CoA-transferase (in reverse): acetate + acetoacetyl-CoA→acetyl-CoA + acetoacetate (EC 2.8.3.9)
No genes
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
Pf6N2E2_1013	+0.1
Pf6N2E2_1046	-0.0
Pf6N2E2_1834	-0.6
Pf6N2E2_1835	-0.1
Pf6N2E2_1840	+0.0
Pf6N2E2_1922	+0.2
Pf6N2E2_1934	-0.0
Pf6N2E2_1935	+0.6
Pf6N2E2_2290	N.D.
Pf6N2E2_2773	+0.1
Pf6N2E2_3894	+0.2
Pf6N2E2_5493	-0.0
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Butanal dehydrogenase (in reverse): butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
Phosphate butyryltransferase: butanoyl-CoA + phosphate→butanoyl phosphate + coenzyme A (EC 2.3.1.19)
No genes
Branched-chain-fatty-acid kinase (in reverse): butanoyl phosphate + ADP→butanoate + ATP (EC 2.7.2.14; 2.7.2.7)
No genes
Alcohol dehydrogenase (NADP(+)) (in reverse): 1-butanal + NADPH + H+→butan-1-ol + NADP+ (EC 1.1.1.2)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes
Butyrate--acetoacetate CoA-transferase (in reverse): acetoacetyl-CoA + butanoate→acetoacetate + butanoyl-CoA (EC 2.8.3.9)
No genes
Acetoacetate decarboxylase: acetoacetate + H+→acetone + CO2 (EC 4.1.1.4)
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information