MetaCyc Pathway: superpathway of L-threonine metabolism in Klebsiella michiganensis M5al

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Reactions and Genes L-Leucine (N)
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Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
BWI76_RS00980 -0.5
L-threonine + ATP→L-threonine 3-O-phosphate + ADP + H+ (EC 2.7.1.177)
BWI76_RS23205 -0.3
L-threonine 3-dehydrogenase:
L-threonine + NAD+→L-2-amino-3-oxobutanoate + NADH + H+ (EC 1.1.1.103)
BWI76_RS27250 -0.0
L-threonine→acetaldehyde + glycine (EC 4.1.2.48; 4.1.2.5)
BWI76_RS09550 +0.1
Threonine-phosphate decarboxylase:
L-threonine 3-O-phosphate + H+→(R)-1-amino-2-propanol O-2-phosphate + CO2 (EC 4.1.1.81)
BWI76_RS23210 -0.1
Acetaldehyde dehydrogenase (acetylating):
acetaldehyde + coenzyme A + NAD+→acetyl-CoA + NADH + H+ (EC 1.2.1.10)
BWI76_RS02835 -0.1
BWI76_RS03590 -0.0
BWI76_RS16905 +0.2
BWI76_RS17250 +0.1
BWI76_RS20790 +0.0
Glycine C-acetyltransferase (in reverse):
L-2-amino-3-oxobutanoate + coenzyme A→glycine + acetyl-CoA (EC 2.3.1.29)
BWI76_RS27255 +0.1
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
BWI76_RS00980 -0.5
BWI76_RS16730 -0.2
BWI76_RS24210 -0.0
L-threonine 3-dehydrogenase:
L-2-amino-3-oxobutanoate + H+→aminoacetone + CO2 (spontaneous) (EC 1.1.1.103; 1.1.1.381)
BWI76_RS27250 -0.0
Primary-amine oxidase:
aminoacetone + dioxygen + H2O→methylglyoxal + ammonium + hydrogen peroxide (EC 1.4.3.21)
BWI76_RS13080 +0.3
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
BWI76_RS00980 -0.5
BWI76_RS02985 +0.3
BWI76_RS16730 -0.2
BWI76_RS24210 -0.0
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
BWI76_RS00960 -0.2
BWI76_RS00965 -2.4
BWI76_RS04695 +0.1
BWI76_RS04700 +0.4
BWI76_RS12900 -0.1
BWI76_RS16750 -0.0
BWI76_RS27810 -0.1
BWI76_RS27815 +0.2
Formate C-acetyltransferase (in reverse):
2-oxobutanoate + coenzyme A→propanoyl-CoA + formate (EC 2.3.1.54)
BWI76_RS03575 -0.1
BWI76_RS09070 +0.2
BWI76_RS09735 -0.0
BWI76_RS10660 +0.1
BWI76_RS21375 +0.5
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
BWI76_RS00990 -1.0
propanoyl-CoA + phosphate→propanoyl phosphate + coenzyme A (EC 2.3.1.222)
BWI76_RS03560 -0.1
BWI76_RS20240 N.D.
BWI76_RS23150 +0.0
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
BWI76_RS00975 -3.7
Acetate kinase (in reverse):
propanoyl phosphate + ADP→propanoate + ATP (EC 2.7.2.1; 2.7.2.14; 2.7.2.15; 2.7.2.7)
BWI76_RS20235 N.D.
BWI76_RS23200 -0.0
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
BWI76_RS00970 -1.4
BWI76_RS10365 +0.5
BWI76_RS24235 -1.6

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information