MetaCyc Pathway: L-leucine biosynthesis in Klebsiella michiganensis M5al

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Reactions and Genes L-Leucine (N)
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2-isopropylmalate synthase:
3-methyl-2-oxobutanoate + acetyl-CoA + H2O→(2S)-2-isopropylmalate + coenzyme A + H+ (EC 2.3.3.13)
BWI76_RS04680 +0.2
BWI76_RS22105 -0.0
3-isopropylmalate dehydratase (in reverse):
(2S)-2-isopropylmalate→2-isopropylmaleate + H2O (EC 4.2.1.33)
BWI76_RS04665 +0.1
BWI76_RS04670 -0.2
3-isopropylmalate dehydratase (in reverse):
2-isopropylmaleate + H2O→(2R,3S)-3-isopropylmalate (EC 4.2.1.33)
BWI76_RS04665 +0.1
BWI76_RS04670 -0.2
3-isopropylmalate dehydrogenase:
(2R,3S)-3-isopropylmalate + NAD+→(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ (EC 1.1.1.85)
BWI76_RS04675 -0.1
3-isopropylmalate dehydrogenase:
(2S)-2-isopropyl-3-oxosuccinate + H+→4-methyl-2-oxopentanoate + CO2 (spontaneous) (EC 1.1.1.85)
BWI76_RS04675 -0.1
Branched-chain-amino-acid transaminase (in reverse):
4-methyl-2-oxopentanoate + L-glutamate→L-leucine + 2-oxoglutarate (EC 2.6.1.42; 2.6.1.6)
BWI76_RS00970 -1.4
BWI76_RS01925 -0.6
BWI76_RS10365 +0.5
BWI76_RS24235 -1.6

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information