MetaCyc Pathway: 2,4-dinitrotoluene degradation in Pseudomonas fluorescens FW300-N2C3

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Reactions and Genes Uridine (N)
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2,4-dinitrotoluene + NADH + dioxygen→4-methyl-5-nitrocatechol + NAD+ + nitrite (EC 1.14.12.24)
AO356_30135 -0.2
4-methyl-5-nitrocatechol + NAD(P)H + dioxygen→2-hydroxy-5-methylquinone + NAD(P)+ + nitrite + H+ + H2O (EC 1.14.13.210)
No genes
2-hydroxy-5-methylquinone + NADH + 2 H+→2,4,5-trihydroxytoluene + NAD+
No genes
2,4,5-trihydroxytoluene + dioxygen→2,4-dihydroxy-5-methyl-6-oxohexa-2,4-dienoate + H+
No genes
2,4-dihydroxy-5-methyl-6-oxohexa-2,4-dienoate→4-hydroxy-2-keto-5-methyl-6-oxo-3-hexenoate + H+
No genes
4-hydroxy-2-keto-5-methyl-6-oxo-3-hexenoate + H2O→methylmalonate semialdehyde + pyruvate
No genes
Methylmalonate-semialdehyde dehydrogenase (acylating):
methylmalonate semialdehyde + coenzyme A + NAD+ + H2O→propanoyl-CoA + hydrogen carbonate + NADH + H+ (EC 1.2.1.27)
AO356_07870 +0.1
AO356_07950 -1.0
AO356_23175 +0.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information