MetaCyc Pathway: superpathway of fatty acid biosynthesis II (plant) in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes tungstate 0.05 M
remove
acetyl-CoA + a [carboxyl-carrier protein dimer]-N6-carboxybiotinyl-L-lysine→malonyl-CoA + a [biotin carboxyl-carrier-protein dimer]-N6-biotinyl-L-lysine (EC 2.1.3.15)
Pf6N2E2_3053 N.D.
Pf6N2E2_72 N.D.
[Acyl-carrier-protein] S-malonyltransferase:
malonyl-CoA + a soluble [acyl-carrier protein]→a malonyl-[acp] + coenzyme A (EC 2.3.1.39)
Pf6N2E2_4625 +0.1
Pf6N2E2_5951 N.D.
Pf6N2E2_6 +1.1
Beta-ketoacyl-acyl-carrier-protein synthase III:
acetyl-CoA + a malonyl-[acp] + H+→an acetoacetyl-[acp] + CO2 + coenzyme A (EC 2.3.1.180)
Pf6N2E2_2605 +0.9
Pf6N2E2_2693 +0.6
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
an acetoacetyl-[acp] + NADPH + H+→a (3R)-3-hydroxybutanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004 +0.1
Pf6N2E2_1181 -0.5
Pf6N2E2_1257 -0.1
Pf6N2E2_1323 -0.4
Pf6N2E2_1747 +0.3
Pf6N2E2_1925 -0.3
Pf6N2E2_1928 +0.3
Pf6N2E2_3493 -0.7
Pf6N2E2_3704 +0.1
Pf6N2E2_3728 +0.1
Pf6N2E2_383 +0.3
Pf6N2E2_3916 -0.0
Pf6N2E2_394 -0.1
Pf6N2E2_4183 +0.0
Pf6N2E2_5206 +0.2
Pf6N2E2_5209 +0.2
Pf6N2E2_5603 +0.2
Pf6N2E2_5952 N.D.
Pf6N2E2_5967 +0.2
Pf6N2E2_6050 -0.1
Pf6N2E2_686 -0.2
Pf6N2E2_853 -0.1
Pf6N2E2_990 -0.3
Pf6N2E2_995 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxybutanoyl-[acp]→a (2E)-but-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058 N.D.
Pf6N2E2_5871 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-but-2-enoyl-[acp] + NADH + H+→a butanoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433 +0.3
Pf6N2E2_580 N.D.
Beta-ketoacyl-acyl-carrier-protein synthase II:
a butanoyl-[acp] + a malonyl-[acp] + H+→a 3-oxohexanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
Pf6N2E2_1178 +0.6
Pf6N2E2_3726 -0.2
Pf6N2E2_3729 -0.4
Pf6N2E2_5870 N.D.
Pf6N2E2_5954 N.D.
Pf6N2E2_835 +0.2
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxohexanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxyhexanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004 +0.1
Pf6N2E2_1181 -0.5
Pf6N2E2_1257 -0.1
Pf6N2E2_1323 -0.4
Pf6N2E2_1747 +0.3
Pf6N2E2_1925 -0.3
Pf6N2E2_1928 +0.3
Pf6N2E2_3493 -0.7
Pf6N2E2_3704 +0.1
Pf6N2E2_3728 +0.1
Pf6N2E2_383 +0.3
Pf6N2E2_3916 -0.0
Pf6N2E2_394 -0.1
Pf6N2E2_4183 +0.0
Pf6N2E2_5206 +0.2
Pf6N2E2_5209 +0.2
Pf6N2E2_5603 +0.2
Pf6N2E2_5952 N.D.
Pf6N2E2_5967 +0.2
Pf6N2E2_6050 -0.1
Pf6N2E2_686 -0.2
Pf6N2E2_853 -0.1
Pf6N2E2_990 -0.3
Pf6N2E2_995 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyhexanoyl-[acp]→a (2E)-hex-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058 N.D.
Pf6N2E2_5871 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-hex-2-enoyl-[acp] + NADH + H+→a hexanoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433 +0.3
Pf6N2E2_580 N.D.
Beta-ketoacyl-acyl-carrier-protein synthase II:
a hexanoyl-[acp] + a malonyl-[acp] + H+→a 3-oxooctanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
Pf6N2E2_1178 +0.6
Pf6N2E2_3726 -0.2
Pf6N2E2_3729 -0.4
Pf6N2E2_5870 N.D.
Pf6N2E2_5954 N.D.
Pf6N2E2_835 +0.2
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxooctanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxyoctanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004 +0.1
Pf6N2E2_1181 -0.5
Pf6N2E2_1257 -0.1
Pf6N2E2_1323 -0.4
Pf6N2E2_1747 +0.3
Pf6N2E2_1925 -0.3
Pf6N2E2_1928 +0.3
Pf6N2E2_3493 -0.7
Pf6N2E2_3704 +0.1
Pf6N2E2_3728 +0.1
Pf6N2E2_383 +0.3
Pf6N2E2_3916 -0.0
Pf6N2E2_394 -0.1
Pf6N2E2_4183 +0.0
Pf6N2E2_5206 +0.2
Pf6N2E2_5209 +0.2
Pf6N2E2_5603 +0.2
Pf6N2E2_5952 N.D.
Pf6N2E2_5967 +0.2
Pf6N2E2_6050 -0.1
Pf6N2E2_686 -0.2
Pf6N2E2_853 -0.1
Pf6N2E2_990 -0.3
Pf6N2E2_995 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyoctanoyl-[acp]→a (2E)-oct-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058 N.D.
Pf6N2E2_5871 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-oct-2-enoyl-[acp] + NADH + H+→an octanoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433 +0.3
Pf6N2E2_580 N.D.
Beta-ketoacyl-acyl-carrier-protein synthase II:
an octanoyl-[acp] + a malonyl-[acp] + H+→a 3-oxodecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
Pf6N2E2_1178 +0.6
Pf6N2E2_3726 -0.2
Pf6N2E2_3729 -0.4
Pf6N2E2_5870 N.D.
Pf6N2E2_5954 N.D.
Pf6N2E2_835 +0.2
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxodecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxydecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004 +0.1
Pf6N2E2_1181 -0.5
Pf6N2E2_1257 -0.1
Pf6N2E2_1323 -0.4
Pf6N2E2_1747 +0.3
Pf6N2E2_1925 -0.3
Pf6N2E2_1928 +0.3
Pf6N2E2_3493 -0.7
Pf6N2E2_3704 +0.1
Pf6N2E2_3728 +0.1
Pf6N2E2_383 +0.3
Pf6N2E2_3916 -0.0
Pf6N2E2_394 -0.1
Pf6N2E2_4183 +0.0
Pf6N2E2_5206 +0.2
Pf6N2E2_5209 +0.2
Pf6N2E2_5603 +0.2
Pf6N2E2_5952 N.D.
Pf6N2E2_5967 +0.2
Pf6N2E2_6050 -0.1
Pf6N2E2_686 -0.2
Pf6N2E2_853 -0.1
Pf6N2E2_990 -0.3
Pf6N2E2_995 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydecanoyl-[acp]→a (2E)-dec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058 N.D.
Pf6N2E2_5871 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-dec-2-enoyl-[acp] + NADH + H+→a decanoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433 +0.3
Pf6N2E2_580 N.D.
Beta-ketoacyl-acyl-carrier-protein synthase II:
a decanoyl-[acp] + a malonyl-[acp] + H+→a 3-oxododecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
Pf6N2E2_1178 +0.6
Pf6N2E2_3726 -0.2
Pf6N2E2_3729 -0.4
Pf6N2E2_5870 N.D.
Pf6N2E2_5954 N.D.
Pf6N2E2_835 +0.2
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxododecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxydodecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004 +0.1
Pf6N2E2_1181 -0.5
Pf6N2E2_1257 -0.1
Pf6N2E2_1323 -0.4
Pf6N2E2_1747 +0.3
Pf6N2E2_1925 -0.3
Pf6N2E2_1928 +0.3
Pf6N2E2_3493 -0.7
Pf6N2E2_3704 +0.1
Pf6N2E2_3728 +0.1
Pf6N2E2_383 +0.3
Pf6N2E2_3916 -0.0
Pf6N2E2_394 -0.1
Pf6N2E2_4183 +0.0
Pf6N2E2_5206 +0.2
Pf6N2E2_5209 +0.2
Pf6N2E2_5603 +0.2
Pf6N2E2_5952 N.D.
Pf6N2E2_5967 +0.2
Pf6N2E2_6050 -0.1
Pf6N2E2_686 -0.2
Pf6N2E2_853 -0.1
Pf6N2E2_990 -0.3
Pf6N2E2_995 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydodecanoyl-[acp]→a (2E)-dodec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058 N.D.
Pf6N2E2_5871 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-dodec-2-enoyl-[acp] + NADH + H+→a dodecanoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433 +0.3
Pf6N2E2_580 N.D.
Dodecanoyl-[acyl-carrier-protein] hydrolase:
a dodecanoyl-[acp][nil] + H2O[nil]→laurate[nil] + a soluble [acyl-carrier protein][nil] + H+[nil] (EC 3.1.2.21)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a dodecanoyl-[acp] + a malonyl-[acp] + H+→a 3-oxotetradecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
Pf6N2E2_1178 +0.6
Pf6N2E2_3726 -0.2
Pf6N2E2_3729 -0.4
Pf6N2E2_5870 N.D.
Pf6N2E2_5954 N.D.
Pf6N2E2_835 +0.2
Butyrate--CoA ligase:
laurate + ATP + coenzyme A→lauroyl-CoA + AMP + diphosphate (EC 6.2.1.2; 6.2.1.3)
Pf6N2E2_2190 +0.1
Pf6N2E2_2872 +0.9
Pf6N2E2_2873 -0.0
Pf6N2E2_5827 +0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxotetradecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxytetradecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004 +0.1
Pf6N2E2_1181 -0.5
Pf6N2E2_1257 -0.1
Pf6N2E2_1323 -0.4
Pf6N2E2_1747 +0.3
Pf6N2E2_1925 -0.3
Pf6N2E2_1928 +0.3
Pf6N2E2_3493 -0.7
Pf6N2E2_3704 +0.1
Pf6N2E2_3728 +0.1
Pf6N2E2_383 +0.3
Pf6N2E2_3916 -0.0
Pf6N2E2_394 -0.1
Pf6N2E2_4183 +0.0
Pf6N2E2_5206 +0.2
Pf6N2E2_5209 +0.2
Pf6N2E2_5603 +0.2
Pf6N2E2_5952 N.D.
Pf6N2E2_5967 +0.2
Pf6N2E2_6050 -0.1
Pf6N2E2_686 -0.2
Pf6N2E2_853 -0.1
Pf6N2E2_990 -0.3
Pf6N2E2_995 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxytetradecanoyl-[acp]→a (2E)-tetradec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058 N.D.
Pf6N2E2_5871 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-tetradec-2-enoyl-[acp] + NADH + H+→a myristoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433 +0.3
Pf6N2E2_580 N.D.
Beta-ketoacyl-acyl-carrier-protein synthase II:
a myristoyl-[acp] + a malonyl-[acp] + H+→a 3-oxohexadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
Pf6N2E2_1178 +0.6
Pf6N2E2_3726 -0.2
Pf6N2E2_3729 -0.4
Pf6N2E2_5870 N.D.
Pf6N2E2_5954 N.D.
Pf6N2E2_835 +0.2
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxohexadecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxyhexadecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004 +0.1
Pf6N2E2_1181 -0.5
Pf6N2E2_1257 -0.1
Pf6N2E2_1323 -0.4
Pf6N2E2_1747 +0.3
Pf6N2E2_1925 -0.3
Pf6N2E2_1928 +0.3
Pf6N2E2_3493 -0.7
Pf6N2E2_3704 +0.1
Pf6N2E2_3728 +0.1
Pf6N2E2_383 +0.3
Pf6N2E2_3916 -0.0
Pf6N2E2_394 -0.1
Pf6N2E2_4183 +0.0
Pf6N2E2_5206 +0.2
Pf6N2E2_5209 +0.2
Pf6N2E2_5603 +0.2
Pf6N2E2_5952 N.D.
Pf6N2E2_5967 +0.2
Pf6N2E2_6050 -0.1
Pf6N2E2_686 -0.2
Pf6N2E2_853 -0.1
Pf6N2E2_990 -0.3
Pf6N2E2_995 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyhexadecanoyl-[acp]→a (2E)-hexadec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058 N.D.
Pf6N2E2_5871 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-hexadec-2-enoyl-[acp] + NADH + H+→a palmitoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433 +0.3
Pf6N2E2_580 N.D.
Oleoyl-[acyl-carrier-protein] hydrolase:
a palmitoyl-[acp] + H2O→palmitate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase I:
a palmitoyl-[acp] + a malonyl-[acp] + H+→a 3-oxooctadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.41)
Pf6N2E2_1178 +0.6
Pf6N2E2_3726 -0.2
Pf6N2E2_3729 -0.4
Pf6N2E2_5870 N.D.
Long-chain-fatty-acid--CoA ligase:
palmitate + ATP + coenzyme A→palmitoyl-CoA + AMP + diphosphate (EC 6.2.1.3)
Pf6N2E2_2190 +0.1
Pf6N2E2_2872 +0.9
Pf6N2E2_2873 -0.0
Pf6N2E2_5827 +0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxooctadecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxyoctadecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Pf6N2E2_1004 +0.1
Pf6N2E2_1181 -0.5
Pf6N2E2_1257 -0.1
Pf6N2E2_1323 -0.4
Pf6N2E2_1747 +0.3
Pf6N2E2_1925 -0.3
Pf6N2E2_1928 +0.3
Pf6N2E2_3493 -0.7
Pf6N2E2_3704 +0.1
Pf6N2E2_3728 +0.1
Pf6N2E2_383 +0.3
Pf6N2E2_3916 -0.0
Pf6N2E2_394 -0.1
Pf6N2E2_4183 +0.0
Pf6N2E2_5206 +0.2
Pf6N2E2_5209 +0.2
Pf6N2E2_5603 +0.2
Pf6N2E2_5952 N.D.
Pf6N2E2_5967 +0.2
Pf6N2E2_6050 -0.1
Pf6N2E2_686 -0.2
Pf6N2E2_853 -0.1
Pf6N2E2_990 -0.3
Pf6N2E2_995 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyoctadecanoyl-[acp]→a (2E)-octadec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Pf6N2E2_3058 N.D.
Pf6N2E2_5871 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-octadec-2-enoyl-[acp] + NADH + H+→a stearoyl-[acp] + NAD+ (EC 1.3.1.9)
Pf6N2E2_433 +0.3
Pf6N2E2_580 N.D.
Acyl-[acyl-carrier-protein] desaturase:
a stearoyl-[acp] + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→an oleoyl-[acp] + 2 an oxidized ferredoxin [iron-sulfur] cluster + 2 H2O (EC 1.14.19.2)
No genes
Oleoyl-[acyl-carrier-protein] hydrolase:
a stearoyl-[acp] + H2O→stearate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes
Oleoyl-[acyl-carrier-protein] hydrolase:
an oleoyl-[acp] + H2O→oleate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes
Long-chain-fatty-acid--CoA ligase:
stearate + ATP + coenzyme A→stearoyl-CoA + AMP + diphosphate (EC 6.2.1.3)
Pf6N2E2_2190 +0.1
Pf6N2E2_2872 +0.9
Pf6N2E2_2873 -0.0
Pf6N2E2_5827 +0.0
Long-chain-fatty-acid--CoA ligase:
oleate + ATP + coenzyme A→oleoyl-CoA + AMP + diphosphate (EC 6.2.1.3)
Pf6N2E2_2190 +0.1
Pf6N2E2_2872 +0.9
Pf6N2E2_2873 -0.0
Pf6N2E2_5827 +0.0

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information