MetaCyc Pathway: molybdopterin biosynthesis in Klebsiella michiganensis M5al

Reactions and Genes	L-Threonine (N)
ATP + a small subunit of molybdopterin synthase + H+→a carboxy-adenylated [small subunit of molybdopterin synthase] + diphosphate (EC 2.7.7.80)
BWI76_RS09080	+1.4
Cysteine desulfurase: L-cysteine + a [molybdopterin synthase sulfurtransferase]-L-cysteine→a [molybdopterin synthase sulfurtransferase]-S-sulfanyl-L-cysteine + L-alanine (EC 2.8.1.7)
BWI76_RS16975	-0.1
BWI76_RS16980	-0.2
BWI76_RS18855	-0.9
BWI76_RS21160	N.D.
BWI76_RS22965	+0.1
BWI76_RS22970	N.D.
GTP + a reduced two electron carrier + S-adenosyl-L-methionine→(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + an oxidized electron carrier + 5'-deoxyadenosine + L-methionine + H+ (EC 4.1.99.22)
No genes
a carboxy-adenylated [small subunit of molybdopterin synthase] + a [molybdopterin synthase sulfurtransferase]-S-sulfanyl-L-cysteine + a reduced two electron carrier→a thiocarboxylated [small subunit of molybdopterin synthase] + AMP + an oxidized electron carrier + a [molybdopterin synthase sulfurtransferase]-L-cysteine + H+ (EC 2.8.1.11)
BWI76_RS11630	+0.1
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate→cyclic pyranopterin phosphate + diphosphate (EC 4.6.1.17)
BWI76_RS08825	+1.6
cyclic pyranopterin phosphate + 2 a thiocarboxylated [small subunit of molybdopterin synthase] + H2O→molybdopterin + 2 a small subunit of molybdopterin synthase + 4 H+ (EC 2.8.1.12)
BWI76_RS08830	N.D.
BWI76_RS08835	+1.6

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information