| Reactions and Genes | PYE 0.5x 30C survival rep2 12 days |
|---|
| remove |
Long-chain-fatty-acid--CoA ligase:
oleate + ATP + coenzyme A→oleoyl-CoA + AMP + diphosphate (EC 6.2.1.3) |
| CCNA_00993 | -0.1 |
| CCNA_01017 | -0.2 |
| CCNA_01382 | +0.3 |
| CCNA_02384 | -2.1 |
| oleoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E,9Z)-octadec-2,9-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8) |
| No genes |
Enoyl-CoA hydratase (in reverse):
(2E,9Z)-octadec-2,9-enoyl-CoA + H2O→(S)-3-hydroxy-(9Z)-octadecenoyl-CoA (EC 4.2.1.17) |
| CCNA_00006 | -2.0 |
| CCNA_00074 | -0.1 |
| CCNA_00358 | +0.1 |
| CCNA_00401 | +0.2 |
| CCNA_00996 | +0.1 |
| CCNA_01414 | -4.4 |
| CCNA_01794 | -0.8 |
| CCNA_01890 | +0.0 |
| CCNA_01891 | +0.1 |
| CCNA_01927 | -0.1 |
| CCNA_02484 | -0.2 |
| CCNA_02658 | -1.2 |
| CCNA_03180 | -1.2 |
| CCNA_03293 | -0.3 |
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxy-(9Z)-octadecenoyl-CoA + NAD+→3-oxo-(9Z)-octadecenoyl-CoA + NADH + H+ (EC 1.1.1.35) |
| CCNA_00074 | -0.1 |
| CCNA_01890 | +0.0 |
| CCNA_03293 | -0.3 |
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-(9Z)-octadecenoyl-CoA + coenzyme A→acetyl-CoA + (7Z)-hexadecenoyl-CoA (EC 2.3.1.16) |
| CCNA_00075 | -0.2 |
| CCNA_00266 | -0.0 |
| CCNA_00544 | -0.1 |
| CCNA_00820 | -0.1 |
| CCNA_00938 | +1.0 |
| CCNA_01168 | +0.2 |
| CCNA_01499 | -0.4 |
| CCNA_02490 | +0.5 |
| CCNA_03209 | -0.5 |
| CCNA_03575 | +0.1 |
| (7Z)-hexadecenoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E,7Z)-hexadec-2,7-dienoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8) |
| No genes |
Enoyl-CoA hydratase (in reverse):
(2E,7Z)-hexadec-2,7-dienoyl-CoA + H2O→(S)-3-hydroxy-(7Z)-hexadecenoyl-CoA (EC 4.2.1.17) |
| CCNA_00006 | -2.0 |
| CCNA_00074 | -0.1 |
| CCNA_00358 | +0.1 |
| CCNA_00401 | +0.2 |
| CCNA_00996 | +0.1 |
| CCNA_01414 | -4.4 |
| CCNA_01794 | -0.8 |
| CCNA_01890 | +0.0 |
| CCNA_01891 | +0.1 |
| CCNA_01927 | -0.1 |
| CCNA_02484 | -0.2 |
| CCNA_02658 | -1.2 |
| CCNA_03180 | -1.2 |
| CCNA_03293 | -0.3 |
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxy-(7Z)-hexadecenoyl-CoA + NAD+→3-oxo-(7Z)-hexadecenoyl-CoA + NADH + H+ (EC 1.1.1.35) |
| CCNA_00074 | -0.1 |
| CCNA_01890 | +0.0 |
| CCNA_03293 | -0.3 |
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-(7Z)-hexadecenoyl-CoA + coenzyme A→acetyl-CoA + (5Z)-tetradecenoyl-CoA (EC 2.3.1.16) |
| CCNA_00075 | -0.2 |
| CCNA_00266 | -0.0 |
| CCNA_00544 | -0.1 |
| CCNA_00820 | -0.1 |
| CCNA_00938 | +1.0 |
| CCNA_01168 | +0.2 |
| CCNA_01499 | -0.4 |
| CCNA_02490 | +0.5 |
| CCNA_03209 | -0.5 |
| CCNA_03575 | +0.1 |
| (5Z)-tetradecenoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E,5Z)-tetradec-2,5-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8) |
| No genes |
Dodecenoyl-CoA isomerase:
(2E,5Z)-tetradec-2,5-enoyl-CoA→(3E,5Z)-tetradeca-3,5-dienoyl-CoA (EC 5.3.3.8) |
| CCNA_01890 | +0.0 |
Enoyl-CoA hydratase (in reverse):
(2E,5Z)-tetradec-2,5-enoyl-CoA + H2O→(S)-3-hydroxy-(5Z)-tetradecenoyl-CoA (EC 4.2.1.17) |
| CCNA_00006 | -2.0 |
| CCNA_00074 | -0.1 |
| CCNA_00358 | +0.1 |
| CCNA_00401 | +0.2 |
| CCNA_00996 | +0.1 |
| CCNA_01414 | -4.4 |
| CCNA_01794 | -0.8 |
| CCNA_01890 | +0.0 |
| CCNA_01891 | +0.1 |
| CCNA_01927 | -0.1 |
| CCNA_02484 | -0.2 |
| CCNA_02658 | -1.2 |
| CCNA_03180 | -1.2 |
| CCNA_03293 | -0.3 |
Long-chain-3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxy-(5Z)-tetradecenoyl-CoA + NAD+→3-oxo-(5Z)-tetradecenoyl-CoA + NADH + H+ (EC 1.1.1.211; 1.1.1.35) |
| CCNA_00074 | -0.1 |
| CCNA_01890 | +0.0 |
| CCNA_03293 | -0.3 |
| (3E,5Z)-tetradeca-3,5-dienoyl-CoA + H2O→(3E,5Z)-tetradeca-3,5-dienoate + coenzyme A + H+ |
| CCNA_03764 | -0.3 |
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-(5Z)-tetradecenoyl-CoA + coenzyme A→acetyl-CoA + (3Z)-dodec-3-enoyl-CoA (EC 2.3.1.16) |
| CCNA_00075 | -0.2 |
| CCNA_00266 | -0.0 |
| CCNA_00544 | -0.1 |
| CCNA_00820 | -0.1 |
| CCNA_00938 | +1.0 |
| CCNA_01168 | +0.2 |
| CCNA_01499 | -0.4 |
| CCNA_02490 | +0.5 |
| CCNA_03209 | -0.5 |
| CCNA_03575 | +0.1 |
Dodecenoyl-CoA isomerase:
(3Z)-dodec-3-enoyl-CoA→(2E)-dodec-2-enoyl-CoA (EC 5.3.3.8) |
| CCNA_01890 | +0.0 |
Long-chain-enoyl-CoA hydratase (in reverse):
(2E)-dodec-2-enoyl-CoA + H2O→(S)-3-hydroxydodecanoyl-CoA (EC 4.2.1.74) |
| No genes |
Long-chain-3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxydodecanoyl-CoA + NAD+→3-oxododecanoyl-CoA + NADH + H+ (EC 1.1.1.211; 1.1.1.35) |
| CCNA_00074 | -0.1 |
| CCNA_01890 | +0.0 |
| CCNA_03293 | -0.3 |
Acetyl-CoA C-acyltransferase (in reverse):
3-oxododecanoyl-CoA + coenzyme A→acetyl-CoA + decanoyl-CoA (EC 2.3.1.16) |
| CCNA_00075 | -0.2 |
| CCNA_00266 | -0.0 |
| CCNA_00544 | -0.1 |
| CCNA_00820 | -0.1 |
| CCNA_00938 | +1.0 |
| CCNA_01168 | +0.2 |
| CCNA_01499 | -0.4 |
| CCNA_02490 | +0.5 |
| CCNA_03209 | -0.5 |
| CCNA_03575 | +0.1 |
| decanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-dec-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7) |
| CCNA_00078 | -0.9 |
| CCNA_00726 | -4.2 |
| CCNA_03504 | -0.3 |
Long-chain-enoyl-CoA hydratase (in reverse):
(2E)-dec-2-enoyl-CoA + H2O→(S)-3-hydroxydecanoyl-CoA (EC 4.2.1.74) |
| CCNA_00358 | +0.1 |
| CCNA_00401 | +0.2 |
| CCNA_01794 | -0.8 |
| CCNA_01890 | +0.0 |
| CCNA_01927 | -0.1 |
| CCNA_02484 | -0.2 |
| CCNA_02658 | -1.2 |
| CCNA_03180 | -1.2 |
| CCNA_03293 | -0.3 |
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxydecanoyl-CoA + NAD+→3-oxodecanoyl-CoA + NADH + H+ (EC 1.1.1.35) |
| CCNA_00074 | -0.1 |
| CCNA_01890 | +0.0 |
| CCNA_03293 | -0.3 |
Acetyl-CoA C-acyltransferase (in reverse):
3-oxodecanoyl-CoA + coenzyme A→acetyl-CoA + octanoyl-CoA (EC 2.3.1.16) |
| CCNA_00075 | -0.2 |
| CCNA_00266 | -0.0 |
| CCNA_00544 | -0.1 |
| CCNA_00820 | -0.1 |
| CCNA_00938 | +1.0 |
| CCNA_01168 | +0.2 |
| CCNA_01499 | -0.4 |
| CCNA_02490 | +0.5 |
| CCNA_03209 | -0.5 |
| CCNA_03575 | +0.1 |
| octanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-oct-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7) |
| CCNA_00078 | -0.9 |
| CCNA_00726 | -4.2 |
| CCNA_03504 | -0.3 |
Long-chain-enoyl-CoA hydratase (in reverse):
(2E)-oct-2-enoyl-CoA + H2O→(3S)-3-hydroxyoctanoyl-CoA (EC 4.2.1.74) |
| No genes |
3-hydroxyacyl-CoA dehydrogenase:
(3S)-3-hydroxyoctanoyl-CoA + NAD+→3-oxooctanoyl-CoA + NADH + H+ (EC 1.1.1.35) |
| CCNA_00074 | -0.1 |
| CCNA_01890 | +0.0 |
| CCNA_03293 | -0.3 |
Acetyl-CoA C-acyltransferase (in reverse):
3-oxooctanoyl-CoA + coenzyme A→acetyl-CoA + hexanoyl-CoA (EC 2.3.1.16) |
| CCNA_00075 | -0.2 |
| CCNA_00266 | -0.0 |
| CCNA_00544 | -0.1 |
| CCNA_00820 | -0.1 |
| CCNA_00938 | +1.0 |
| CCNA_01168 | +0.2 |
| CCNA_01499 | -0.4 |
| CCNA_02490 | +0.5 |
| CCNA_03209 | -0.5 |
| CCNA_03575 | +0.1 |
| hexanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-hex-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7) |
| CCNA_00078 | -0.9 |
| CCNA_00726 | -4.2 |
| CCNA_03504 | -0.3 |
Long-chain-enoyl-CoA hydratase (in reverse):
(2E)-hex-2-enoyl-CoA + H2O→(S)-3-hydroxyhexanoyl-CoA (EC 4.2.1.74) |
| CCNA_00358 | +0.1 |
| CCNA_00401 | +0.2 |
| CCNA_01794 | -0.8 |
| CCNA_01890 | +0.0 |
| CCNA_01927 | -0.1 |
| CCNA_02484 | -0.2 |
| CCNA_02658 | -1.2 |
| CCNA_03180 | -1.2 |
| CCNA_03293 | -0.3 |
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxyhexanoyl-CoA + NAD+→3-oxohexanoyl-CoA + NADH + H+ (EC 1.1.1.35) |
| CCNA_00074 | -0.1 |
| CCNA_01890 | +0.0 |
| CCNA_03293 | -0.3 |
Acetyl-CoA C-acyltransferase (in reverse):
3-oxohexanoyl-CoA + coenzyme A→acetyl-CoA + butanoyl-CoA (EC 2.3.1.16; 2.3.1.9) |
| CCNA_00075 | -0.2 |
| CCNA_00266 | -0.0 |
| CCNA_00544 | -0.1 |
| CCNA_00820 | -0.1 |
| CCNA_00938 | +1.0 |
| CCNA_01168 | +0.2 |
| CCNA_01499 | -0.4 |
| CCNA_02045 | +0.1 |
| CCNA_02490 | +0.5 |
| CCNA_03209 | -0.5 |
| CCNA_03575 | +0.1 |
| butanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→crotonyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.1) |
| No genes |
| crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150) |
| CCNA_00358 | +0.1 |
| CCNA_00401 | +0.2 |
| CCNA_01794 | -0.8 |
| CCNA_01890 | +0.0 |
| CCNA_01927 | -0.1 |
| CCNA_02484 | -0.2 |
| CCNA_02658 | -1.2 |
| CCNA_03180 | -1.2 |
| CCNA_03293 | -0.3 |
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35) |
| CCNA_00074 | -0.1 |
| CCNA_00752 | -0.3 |
| CCNA_01890 | +0.0 |
| CCNA_03293 | -0.3 |
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9) |
| CCNA_00075 | -0.2 |
| CCNA_00266 | -0.0 |
| CCNA_00544 | -0.1 |
| CCNA_00820 | -0.1 |
| CCNA_00938 | +1.0 |
| CCNA_01168 | +0.2 |
| CCNA_01499 | -0.4 |
| CCNA_02045 | +0.1 |
| CCNA_02490 | +0.5 |
| CCNA_03209 | -0.5 |
| CCNA_03575 | +0.1 |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information