MetaCyc Pathway: 3-methylbutanol biosynthesis (engineered) in Dechlorosoma suillum PS

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Reactions and Genes propionate (C)
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2-isopropylmalate synthase:
3-methyl-2-oxobutanoate + acetyl-CoA + H2O→(2S)-2-isopropylmalate + coenzyme A + H+ (EC 2.3.3.13)
Dsui_3227 -1.6
Dsui_3519 +0.0
3-isopropylmalate dehydratase (in reverse):
(2S)-2-isopropylmalate→2-isopropylmaleate + H2O (EC 4.2.1.33)
Dsui_3199 N.D.
Dsui_3201 N.D.
3-isopropylmalate dehydratase (in reverse):
2-isopropylmaleate + H2O→(2R,3S)-3-isopropylmalate (EC 4.2.1.33)
Dsui_3199 N.D.
Dsui_3201 N.D.
3-isopropylmalate dehydrogenase:
(2R,3S)-3-isopropylmalate + NAD+→(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ (EC 1.1.1.85)
Dsui_3198 N.D.
3-isopropylmalate dehydrogenase:
(2S)-2-isopropyl-3-oxosuccinate + H+→4-methyl-2-oxopentanoate + CO2 (spontaneous) (EC 1.1.1.85)
Dsui_3198 N.D.
Branched-chain-2-oxoacid decarboxylase:
4-methyl-2-oxopentanoate + H+→3-methylbutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
3-methylbutanal + NADH + H+→3-methylbutanol + NAD+ (EC 1.1.1.1)
Dsui_2228 -1.7

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information