MetaCyc Pathway: benzoate fermentation (to acetate and cyclohexane carboxylate) in Paraburkholderia bryophila 376MFSha3.1

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Reactions and Genes Phenylacetic (C)
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Benzoate--CoA ligase:
benzoate + ATP + coenzyme A→benzoyl-CoA + AMP + diphosphate (EC 6.2.1.25)
H281DRAFT_00369 -0.1
benzoyl-CoA + 2 ATP + 2 a reduced ferredoxin [iron-sulfur] cluster + 2 H2O→cyclohex-1,5-diene-1-carbonyl-CoA + 2 ADP + 2 an oxidized ferredoxin [iron-sulfur] cluster + 2 phosphate (EC 1.3.7.8)
No genes
Cyclohexa-1,5-dienecarbonyl-CoA hydratase (in reverse):
cyclohex-1,5-diene-1-carbonyl-CoA + H2O→6-hydroxycyclohex-1-ene-1-carbonyl-CoA (EC 4.2.1.100)
No genes
cyclohex-1,5-diene-1-carbonyl-CoA + a reduced electron-transfer flavoprotein→cyclohex-1-ene-1-carbonyl-CoA + an oxidized electron-transfer flavoprotein + H+ (EC 1.3.8.10)
No genes
6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+→6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H+ (EC 1.1.1.368)
No genes
cyclohex-1-ene-1-carbonyl-CoA + a reduced electron-transfer flavoprotein→cyclohexane-1-carbonyl-CoA + an oxidized electron-transfer flavoprotein + H+ (EC 1.3.8.11)
No genes
cyclohexane-1-carbonyl-CoA + H2O→cyclohexane-1-carboxylate + coenzyme A + H+
No genes
6-oxocyclohex-1-ene-1-carbonyl-CoA + H2O→2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA (EC 3.7.1.21)
No genes
2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA + H2O→3-hydroxypimeloyl-CoA + H+ (EC 3.7.1.21)
No genes
3-hydroxypimeloyl-CoA dehydrogenase:
3-hydroxypimeloyl-CoA + NAD+→3-oxopimeloyl-CoA + NADH + H+ (EC 1.1.1.259)
No genes
3-oxopimeloyl-CoA + coenzyme A→glutaryl-CoA + acetyl-CoA
H281DRAFT_00917 +0.3
glutaryl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-glutaconyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.6)
No genes
(E)-glutaconyl-CoA + H+→crotonyl-CoA + CO2 (EC 1.3.8.6)
No genes
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
H281DRAFT_00361 -1.8
H281DRAFT_00653 +0.7
H281DRAFT_00756 -0.1
H281DRAFT_00916 -0.2
H281DRAFT_01137 N.D.
H281DRAFT_01179 -0.1
H281DRAFT_01187 +0.1
H281DRAFT_01670 +0.2
H281DRAFT_01774 N.D.
H281DRAFT_02068 -0.5
H281DRAFT_02497 -0.7
H281DRAFT_02514 -0.2
H281DRAFT_02515 N.D.
H281DRAFT_02677 +0.0
H281DRAFT_02741 +0.3
H281DRAFT_02927 +0.2
H281DRAFT_03491 N.D.
H281DRAFT_03701 +0.3
H281DRAFT_03739 +0.1
H281DRAFT_03908 -0.0
H281DRAFT_04625 -0.5
H281DRAFT_04627 -0.3
H281DRAFT_04744 -0.1
H281DRAFT_05516 +0.2
H281DRAFT_05977 -0.1
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
H281DRAFT_00361 -1.8
H281DRAFT_00916 -0.2
H281DRAFT_03517 -0.5
H281DRAFT_04595 -0.9
H281DRAFT_04625 -0.5
H281DRAFT_05521 +0.8
H281DRAFT_06131 -0.2
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
H281DRAFT_00852 -1.6
H281DRAFT_00857 -0.1
H281DRAFT_00917 +0.3
H281DRAFT_01504 +0.1
H281DRAFT_02089 -0.1
H281DRAFT_04626 -0.2
H281DRAFT_05130 N.D.
H281DRAFT_05518 -0.1
H281DRAFT_05559 +0.0
H281DRAFT_05723 -5.2
H281DRAFT_06132 +0.1
Acetate--CoA ligase (ADP-forming) (in reverse):
acetyl-CoA + ADP + phosphate→acetate + ATP + coenzyme A (EC 6.2.1.13)
H281DRAFT_03009 +0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information