MetaCyc Pathway: Bifidobacterium shunt in Bifidobacterium breve UCC2003

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Reactions and Genes reconstituted MRS with ISOThrive-prebiotic lot A 0.5 %w/v
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Hexokinase:
D-glucopyranose + ATP→D-glucopyranose 6-phosphate + ADP + H+ (EC 2.7.1.1; 2.7.1.2)
BBR_RS11100 +0.1
BBR_RS18130 -1.4
BBR_RS18495 +0.5
Glucose-6-phosphate isomerase:
α-D-glucose 6-phosphate→β-D-fructofuranose 6-phosphate (EC 5.3.1.9)
BBR_RS12430 N.D.
Fructose-6-phosphate phosphoketolase:
β-D-fructofuranose 6-phosphate + phosphate→acetyl phosphate + D-erythrose 4-phosphate + H2O (EC 4.1.2.22)
BBR_RS14410 N.D.
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
BBR_RS14375 N.D.
Transaldolase (in reverse):
D-erythrose 4-phosphate + β-D-fructofuranose 6-phosphate→D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate (EC 2.2.1.2)
BBR_RS15575 N.D.
Transketolase:
D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate→D-ribose 5-phosphate + D-xylulose 5-phosphate (EC 2.2.1.1)
BBR_RS15580 N.D.
Ribose-5-phosphate isomerase:
D-ribose 5-phosphate→D-ribulose 5-phosphate (EC 5.3.1.6)
BBR_RS18730 N.D.
Ribulose-phosphate 3-epimerase:
D-ribulose 5-phosphate→D-xylulose 5-phosphate (EC 5.1.3.1)
BBR_RS15800 N.D.
BBR_RS17570 +0.1
Phosphoketolase:
D-xylulose 5-phosphate + phosphate→acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O (EC 4.1.2.9)
BBR_RS14410 N.D.
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
BBR_RS16790 N.D.
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
BBR_RS15535 N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
BBR_RS14425 +0.2
BBR_RS18590 N.D.
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
BBR_RS14125 N.D.
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
BBR_RS14295 N.D.
L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(S)-lactate + NAD+ (EC 1.1.1.27)
BBR_RS15550 +0.1
BBR_RS16995 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information