MetaCyc Pathway: partial TCA cycle (obligate autotrophs) in Pseudomonas stutzeri RCH2

Add experiment(s):


Reactions and Genes Def Chloride 400 mM
remove
Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate
(EC 4.1.1.31)
Psest_1557 -2.5
Citrate (Si)-synthase:
oxaloacetate + acetyl-CoA + H2O→citrate + coenzyme A + H+
(EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
Psest_2499 N.D.
oxaloacetate[in] + an electron-transfer quinol→(S)-malate[in] + an electron-transfer quinone (EC 1.1.5.4)
Psest_3238 N.D.
Aconitate hydratase:
citrate→cis-aconitate + H2O
(EC 4.2.1.3)
Psest_2309 N.D.
Psest_2319 N.D.
Psest_2521 -0.2
Fumarate hydratase:
(S)-malate→fumarate + H2O
(EC 4.2.1.2)
Psest_2682 +0.1
Psest_3193 N.D.
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate
(EC 4.2.1.3)
Psest_2309 N.D.
Psest_2319 N.D.
Psest_2521 -0.2
Succinate dehydrogenase (ubiquinone) (in reverse):
fumarate[in] + an electron-transfer quinol→succinate[in] + an electron-transfer quinone
(EC 1.3.5.1)
Psest_2495 N.D.
Psest_2496 N.D.
Psest_2497 N.D.
Psest_2498 N.D.
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→2-oxoglutarate + CO2 + NADPH
(EC 1.1.1.42)
Psest_2023 +0.5
Psest_2024 +0.3

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information