MetaCyc Pathway: L-methionine biosynthesis II in Enterobacter sp. TBS_079

Reactions and Genes	TSB plate, 1:1 coculture with Microbacterium trichothecenolyticum CFG_272
Homoserine kinase: L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
MPMX20_00711	-2.6
Cystathionine gamma-synthase: O-phospho-L-homoserine + L-cysteine→L-cystathionine + phosphate (EC 2.5.1.48)
MPMX20_04531	-2.1
Cysteine-S-conjugate beta-lyase: L-cystathionine→2-aminoprop-2-enoate + L-homocysteine (EC 4.4.1.13)
No genes
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase: L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate (EC 2.1.1.14)
MPMX20_02184	-0.4
MPMX20_02249	-0.7
MPMX20_04451	-1.3
Tryptophanase: 2-aminoprop-2-enoate→2-iminopropanoate (spontaneous) (EC 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
MPMX20_00023	+0.2
MPMX20_01953	+0.2
MPMX20_02719	+0.3
MPMX20_02844	-0.0
MPMX20_03667	+0.2
2-iminopropanoate + H2O→pyruvate + ammonium (spontaneous) (EC 3.5.99.10; 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
MPMX20_00023	+0.2
MPMX20_00542	-0.5
MPMX20_01953	+0.2
MPMX20_02719	+0.3
MPMX20_02844	-0.0
MPMX20_03667	+0.2

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information