MetaCyc Pathway: superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation in Pseudomonas fluorescens FW300-N2C3

Reactions and Genes	Carnitine Hydrochloride (N)
Pyruvate synthase: pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Acetaldehyde dehydrogenase (acetylating) (in reverse): acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
AO356_28020	-0.2
Acetyl-CoA C-acyltransferase: 2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
AO356_02325	-1.5
AO356_03560	-0.1
AO356_04930	-0.4
AO356_08090	+0.1
AO356_20395	-0.0
AO356_21640	-1.7
AO356_26350	+0.4
AO356_30300	-0.2
Phosphate acetyltransferase: acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
AO356_16680	-0.2
Acetate kinase (in reverse): acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
AO356_17605	-0.3
Alcohol dehydrogenase (in reverse): acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
AO356_00820	+0.1
AO356_04765	-0.2
AO356_05920	-1.5
AO356_14405	+0.0
AO356_20305	+0.0
AO356_28020	-0.2
AO356_29985	+0.1
3-hydroxyacyl-CoA dehydrogenase (in reverse): acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
AO356_02330	N.D.
AO356_10120	+0.0
AO356_13845	-3.3
AO356_20400	-0.1
AO356_29995	-0.1
AO356_30295	+0.0
Butyrate--acetoacetate CoA-transferase (in reverse): acetate + acetoacetyl-CoA→acetyl-CoA + acetoacetate (EC 2.8.3.9)
No genes
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
AO356_02330	N.D.
AO356_04640	-0.2
AO356_10120	+0.0
AO356_12440	-0.1
AO356_17825	-0.3
AO356_26645	-0.1
AO356_30295	+0.0
AO356_30355	-0.3
AO356_30360	-0.2
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Butanal dehydrogenase (in reverse): butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
Phosphate butyryltransferase: butanoyl-CoA + phosphate→butanoyl phosphate + coenzyme A (EC 2.3.1.19)
No genes
Branched-chain-fatty-acid kinase (in reverse): butanoyl phosphate + ADP→butanoate + ATP (EC 2.7.2.14; 2.7.2.7)
No genes
Alcohol dehydrogenase (NADP(+)) (in reverse): 1-butanal + NADPH + H+→butan-1-ol + NADP+ (EC 1.1.1.2)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes
Butyrate--acetoacetate CoA-transferase (in reverse): acetoacetyl-CoA + butanoate→acetoacetate + butanoyl-CoA (EC 2.8.3.9)
No genes
Acetoacetate decarboxylase: acetoacetate + H+→acetone + CO2 (EC 4.1.1.4)
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information