MetaCyc Pathway: 3-phenylpropanoate degradation in Pseudomonas fluorescens FW300-N2C3

Reactions and Genes	Carnitine Hydrochloride (N)
3-phenylpropanoate + ATP + coenzyme A→3-phenylpropanoyl-CoA + AMP + diphosphate
No genes
3-phenylpropanoyl-CoA + an oxidized electron carrier→(E)-cinnamoyl-CoA + a reduced two electron carrier
No genes
Enoyl-CoA hydratase (in reverse): (E)-cinnamoyl-CoA + H2O→(S)-3-hydroxy-3-phenylpropanoyl-CoA (EC 4.2.1.17)
AO356_02330	N.D.
AO356_04640	-0.2
AO356_10120	+0.0
AO356_12440	-0.1
AO356_17825	-0.3
AO356_20400	-0.1
AO356_20420	N.D.
AO356_25890	+0.0
AO356_26360	+0.2
AO356_26370	+0.2
AO356_26645	-0.1
AO356_27920	+0.3
AO356_30295	+0.0
AO356_30355	-0.3
AO356_30360	-0.2
3-hydroxyacyl-CoA dehydrogenase: (S)-3-hydroxy-3-phenylpropanoyl-CoA + NAD+→3-oxo-3-phenylpropanoyl-CoA + NADH + H+ (EC 1.1.1.35)
AO356_02330	N.D.
AO356_10120	+0.0
AO356_13845	-3.3
AO356_20400	-0.1
AO356_30295	+0.0
Acetyl-CoA C-acyltransferase (in reverse): 3-oxo-3-phenylpropanoyl-CoA + coenzyme A→benzoyl-CoA + acetyl-CoA (EC 2.3.1.16)
AO356_02325	-1.5
AO356_03560	-0.1
AO356_04930	-0.4
AO356_08090	+0.1
AO356_20395	-0.0
AO356_21640	-1.7
AO356_26350	+0.4
Benzoyl-CoA 3-monooxygenase: benzoyl-CoA + NADPH + dioxygen + H+→3-hydroxybenzoyl-CoA + NADP+ + H2O (EC 1.14.13.58)
No genes
3-hydroxybenzoyl-CoA + a reduced two electron carrier + dioxygen→gentisate + an oxidized electron carrier + coenzyme A + H+
No genes
Gentisate 1,2-dioxygenase: gentisate + dioxygen→3-maleylpyruvate + H+ (EC 1.13.11.4)
No genes
Maleylpyruvate isomerase: 3-maleylpyruvate→3-fumarylpyruvate (EC 5.2.1.4)
AO356_16835	+0.1
3-fumarylpyruvate + H2O→fumarate + pyruvate + H+ (EC 3.7.1.20)
AO356_20665	-0.0

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information