MetaCyc Pathway: stigma estolide biosynthesis in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Nickel (II) chloride 0.0025 M
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oleoyl-CoA + dioxygen + a reduced [NADPH-hemoprotein reductase]→18-hydroxyoleoyl-CoA + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.129)
No genes
linoleoyl-CoA + dioxygen + a reduced [NADPH-hemoprotein reductase]→18-hydroxylinoleoyl-CoA + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.129)
No genes
Glycerol-3-phosphate O-acyltransferase:
18-hydroxyoleoyl-CoA + sn-glycerol 3-phosphate→1-(18-hydroxyoleoyl)-sn-glycrol 3-phosphate + coenzyme A (EC 2.3.1.15)
Pf6N2E2_3087 -0.2
Pf6N2E2_4795 +3.5
Pf6N2E2_5950 -0.2
1-acylglycerol-3-phosphate O-acyltransferase:
18-hydroxylinoleoyl-CoA + 1-(18-hydroxyoleoyl)-sn-glycrol 3-phosphate→1-[18-hydroxyoeoyl]-2-[18-hydroxy-lioleoyl]-sn-glycerol 3-phosphate + coenzyme A (EC 2.3.1.51)
Pf6N2E2_2816 N.D.
Pf6N2E2_4192 N.D.
Pf6N2E2_4950 +0.3
Pf6N2E2_5765 +0.2
Pf6N2E2_6003 +0.1
Phosphatidate phosphatase:
1-[18-hydroxyoeoyl]-2-[18-hydroxy-lioleoyl]-sn-glycerol 3-phosphate + H2O→1-[18-hydroxyoeoyl]-2-[18-hydroxy-linoleoyl]-sn-glycerol + phosphate (EC 3.1.3.4)
No genes
18-hydroxyoleoyl-CoA + 1-[18-hydroxyoeoyl]-2-[18-hydroxy-linoleoyl]-sn-glycerol→tri-acyl estolide + coenzyme A
No genes
18-hydroxylinoleoyl-CoA + tri-acyl estolide→tetra-acyl estolide + coenzyme A
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information