MetaCyc Pathway: NAD de novo biosynthesis I in Pseudomonas fluorescens FW300-N2C3

Reactions and Genes	L-Phenylalanine (N)
L-aspartate oxidase: L-aspartate + dioxygen→2-iminosuccinate + hydrogen peroxide + H+ (EC 1.4.3.16)
AO356_04580	+0.2
Quinolinate synthase: 2-iminosuccinate + glycerone phosphate→quinolinate + phosphate + 2 H2O (EC 2.5.1.72)
AO356_18145	-0.2
Nicotinate-nucleotide diphosphorylase (carboxylating) (in reverse): quinolinate + 2 H+ + 5-phospho-α-D-ribose 1-diphosphate→β-nicotinate D-ribonucleotide + CO2 + diphosphate (EC 2.4.2.19)
AO356_07370	+0.1
Nicotinate-nucleotide adenylyltransferase: β-nicotinate D-ribonucleotide + ATP + H+→nicotinate adenine dinucleotide + diphosphate (EC 2.7.7.18)
AO356_11760	+0.2
AO356_15295	-0.5
NAD(+) synthase (glutamine-hydrolyzing): nicotinate adenine dinucleotide + ATP + L-glutamine + H2O→NAD+ + AMP + L-glutamate + diphosphate + H+ (EC 6.3.5.1)
No genes
NAD(+) synthase: nicotinate adenine dinucleotide + ammonium + ATP→NAD+ + AMP + diphosphate + H+ (EC 6.3.1.5)
AO356_08525	-2.3
AO356_11775	-0.1

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information